Figure 5

EIF2AK2 is required for maintenance of normal 12E8 tau levels and total tau expression. Shown in A is the kinome library screening result for EIF2AK2 siRNA. Effects on 12E8 tau (red) and total tau (green) are shown to the right of each image. In B, triplicate western analyses confirm that EIF2AK2 is required for maintenance of tau and 12E8 tau levels. The EIF2AK2 siRNA reduced expression of EIF2AK2 by 95%, resulting in an 85% reduction of 12E8 tau and a greater than 50% reduction of total tau levels. NS is the non-silencing control siRNA.