Figure 8
From: Reconstruction of xylose utilization pathway and regulons in Firmicutes
EMSA to assess the interactions of C. acetobutylicum XylR with their cognate DNA signals. Each of the three 180-bp target DNA fragments (1 nM) from the upstream region of CAC2611-xylA-II, xylB, and xylR genes, respectively, was incubated for 20 min at 28°C with or without 0.5-0.7 μM of the XylR protein. Specificity of the XylR-DNA interactions was tested by competition with 0.4 μM of non-biotinylated target DNA (specific competitor). Salmon sperm DNA (2 μg) was added to all binding reaction mixtures as a non-specific competitor.