Table 7 Primer sequences for qRT-PCR validation
Gene name (Symbol) | Forward | Reverse | Amplicon size (bp) | Accession number |
|---|---|---|---|---|
B2M1 | TGGTCTTTCTACCTTCTGGTCC | TGTGATGCCGGTTAGTGGTCTC | 134 | NM_004048 |
CD69 | TCCTATCCCATGTGCTGTGGT | GCACATATTGGCCTGGACAGT | 104 | NM_214091 |
CST2 | TCTTGGAAGTGGAGATTGGCC | CACAGCGTTTTCTTCTGCAGG | 103 | BG609449 |
IL1A | GTGTGGTGATGGCAGCAGC | GGTCGTCATCGGTGATGAACT | 81 | NM_214029 |
PSMB9 | GCGCTTCACCACAAATGCTA | TCCACACCAGCAGCTGTAATG | 96 | NM_001037961 |
PSMB8 | TACCTGCTTGGCACCATGTCT | AGTACAACCTGCACTCCTTGGC | 82 | NM_213935 |
LYZ | TCTTGCGCTTCTCCTCCTTTC | AAACATACCCAGTTCGCCAGG | 129 | NM_214392 |
PPIA | GTCTCCTTCGAGCTGTTTGCA | CCAAATCCTTTCTCCCCAGTG | 83 | NM_214353 |
SLAIa | CATCATTGTTGGCCTGGTTC | CCTTTTTCACCTGAGCGC | 90 | NM_001097431(SLA-1) NM_001113702(SLA-2) NM_001097427(SLA-3) |
SLA-DQB | AGGAGATGTCTACACCTGCCG | ATTCAGACTGTGCCCGCC | 80 | NM_001113694 |
SLA-DRA | ATGGGCTATCGTAGAGAATCACG | CAAACATAAACTCGCCAGATTTG | 80 | NM_001113706 |
SLA-DRB1 | CACAGTGGAATGGAGGGCAC | AGCAGACCCAGGACGAAGC | 81 | NM_001113695 |
TAP1 | CCAGTATCTCAGGGATGTTGCTG | CGCTGCTTATAGCCCCACC | 81 | NM_001044581 |
TAP2 | TGTTGGGTGAGACACTAATCCCTTA | CAAAGGCATCAGGGTCAAAATC | 81 | DQ227991 |
TNFRSF9 | TCCAGGTCACACTTCCCATGT | GAACAACAGAGAAACGGAGCG | 101 | BE233113 |