Term used | Definition |
---|---|
Reads | Sequences passing quality control (QC) criteria after BaseCall, generated from 454 sequencing using manufacturer specifications. |
Sequences | Reads remaining in the dataset after the first step of our data processing procedure (Table 2). |
Variants | Sequences differing by at least one base pair substitution or by an indel. |
Artifactual sequences or artifactual variants | Sequences or variants that resulted from sequencing errors, polymerase errors and non-specific amplifications of paralogue and pseudogene during PCR (Table 2). |
True sequences or true variants | Sequences or variants that were retained after validation at all stages of our stepwise procedure. |