Regulation of the cardiomyocyte transcriptome vs translatome by endothelin-1 and insulin: translational regulation of 5' terminal oligopyrimidine tract (TOP) mRNAs by insulin

Table 1 Identification of novel TOP mRNAs.

Gene Symbol	Polysome RNA	Total RNA	5' UTR
Atg12	1.28	1.03	Mouse: *CTTCC* GCCGCCGCCTCTCAGCAAGCAAAGATG Rat: *CTTCC* GCCGTCGACGCTCAGCAAGCAAGATG
Atp5g2	1.26	1.00	Mouse: *CCCTCTCT* GTCTTCTCTGCCCTGGGAGCAGCCCTCCTGCCTCGGCCCCTCACCCCTGAAAATG Rat: *CCCTCTCT* GTCTTCTCTACCCTGGGAGCAGCTCTTCTGCCTTTGCCCCTCACCCCTGAAAAATG
Ccnj	1.52	1.20	Mouse: *CTTCC* AGACTGAAGTTGCGGCGTCTGGCCGGGCGCACCTCCGGCTTCCATG Rat: *CTTCC* AGACTGAAGTTGCGGCGTCTGGCTGGGCGCGCCTCCGGCTTCCATG
Cnih	1.25	1.02	Mouse: *CTTTCTCC* GCTGGCCCCGGCGCGCCCGGCAGCTCCTCCCCGGCCATG Rat: *CTTTCTCC* GCTGGCCCCGGCGCGCCCGGCAGCTCCTCCCCGGCCATG
Gltscr2	1.28	0.91	Mouse: *CTTCCTTT* AAGAAGATG Rat: *CTTCCTTT* AAGAAGATG
Naca	1.25	0.96	Mouse: *CTCTTTCT* GCCGCCATCTTGGTTCCGTGATCTCCGCACAAAATG Rat: *CTCTTTCC* ACCGCCATCTTGGTTCCGTGTTCTCCGCACAAAATG
Pfdn5	1.28	0.90	Mouse: *CTTCCTCTTC* GGCAGTCCTCCTTCCCAACATG Rat: *CTTCC* GCTTCGGCATTCCTCCTTCCCAACATG
Rbms2	1.27	0.96	Mouse: *CTCCCTCCCTTTCCTTC* ACTCTCTTCTTTCTCTCTCTCAGCTCCGTAACAGTAAAGAAAAAATG Rat: *CTCCCTCCCTTTCCTTC* ACTCTCTTCTTTCTCTCTCTCAGCTCCGTAACAGTAAAGAAAAAATG

Total and polysomal RNA from cardiomyocytes exposed to insulin (50 mU/ml, 1 h) or unstimulated cardiomyocytes (controls) were analysed using microarrays. Probesets corresponding to mRNAs with >1.25-fold increase in expression (FDR < 0.05) were identified. Results are the mean fold change in insulin stimulated samples relative to controls. Mouse and rat 5' UTR sequences were identified. The TOP sequence is in bold italics. Accession numbers are provided in Additional data file 6.

ISSN: 1471-2164