Figure 4

FACS analysis of A. fumigatus conidia and epithelial cells incubated alone or together. Cultures of GFP-expressing A. fumigatus conidia alone (A), 16HBE14o- cells alone (B), or cells and conidia incubated together for 6 hrs (C) were analyzed by flow cytometry, using forward scatter (FSC) and side scatter (SSC). The gates defining conidia (labeled 'spores', coloured green) and 16HBE14o- cells (labeled 'cells', coloured blue and purple) were set manually based on the observed distributions by FSC and SSC. Histograms showing the FITC channel signal intensity from the selected gates for (D) conidia alone, (E) 16HBE14o- cells alone, and (F) co-cultures of 16HBE14o- cells and conidia. The bimodal distribution of FITC intensity allowed the sorting of 16HBE14o- cells positive for GFP from negative cells (Figure 4G).