Figure 3
From: Transcriptomic responses in mouse brain exposed to chronic excess of the neurotransmitter glutamate
Up-regulation of PTK2B detected by immunolabelling in the hippocampal CA1 region of Glud1 Tg mouse brain. Antibodies against PTK2B, phosphorylated PTK2B (PTK2BpY402), and MAP2A were used to label reactive neurons in the hippocampal CA1 region of 11 mo Glud1 (A-D) and wt (E-H) mice. Immunolabelled sections were examined under confocal microscopy as described under Methods. Compared with hippocampus neurons in wt mouse, those in the Glud1 Tg mouse exhibited a substantial decrease in dendritic labelling by MAP2A (see also text). On the other hand, labelling by PTK2BpY402 in Tg mouse hippocampus appeared to be higher than that observed in wt mice. After normalization of PTK2BpY402 immunoreactivity by expressing it as a ratio of PTK2BpY402 to MAP2A labelling, the levels of PTK2BpY402 were significantly higher in the Glud1 Tg neurons (see also text), a possible sign of increase in neurite growth in neurons of Tg mice. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bars: 10 μm.