Figure 3

Amino acid alignment of the region between transmembrane (TM) regions 7 and 9 of the α sub-unit of SEC61. Putative cold-adapted amino acid substitutions are indicated at positions 327, 328 and 339. Invertebrate-specific substitutions are labelled in red on the consensus line, insect-specific substitutions labelled in blue and the single potential bivalve-specific substitution labelled in green. Species abbreviations and accession numbers: Nan: Notothenia angustrata (Q8AY35); Pbo: Pagothenia borchgrevinki (Q8AY36); Dma: Dissostichus mawsoni (AY113841); Han: Harpagifer antarcticus (Q7T278); Ham: Hemitripterus americanus (Q8AY34); Bsa: Boreogadus saida (Q8AY33); Gog: Gadus ogac (Q8AY32) (all cold-adapted); Omy: Onchorhynchus mykiss (Q98SN9); Dre: Danio rerio (Q90ZM2); Mmu: Mus musculus (P61620); Cqu: Culex quinquefasciatus (B0WNA0); Aae: Aedes aegypti (Q17CM3); Dme: Drosophila melanogaster (Q8STG9). Lgi: Lottia gigantea (cluster: >jgi|Lotgi1|194715|estExt_Genewise1.C_sca_610223 from sequences: 4236761:1772, 4236761:2059 and 4236761:4476 extracted from http://genome.jgi-psf.org/Lotgi1/Lotgi1.home.html; Lel: Laternula elliptica, contig00029.