Figure 7

Genomic sequence surrounding coding exons 4 and 5 in mouse Doc2g and the equivalent human region. Genomic sequence surrounding coding exons 4 and 5 of mouse Doc2g and the equivalent region in human, are shown. Exonic and intronic sequences are indicated. The reading frame is indicated by grey bars between codons. The single nucleotide deletion in the human exon 5 region is highlighted with a red exclamation mark. The deletion in human exon 5 disrupts the reading frame, leading to a premature termination codon which is boxed and highlighted with a red exclamation mark. An alternative splice acceptor dinucleotide which could restore the correct reading frame to human exon 5 is indicated by a blue arrow. Blue bars between the first few codons indicate the reading frame were the alternative splice acceptor to be functional. Human exon 5 has an alternative start codon, which is boxed and highlighted with a question mark. There is a possibility that this start codon could allow normally spliced transcripts to be translated into the N-terminal and C-terminal protein products listed in additional file 50.