Genome size evolution in pufferfish: an insight from BAC clone-based Diodon holocanthus genome sequencing

BMC Genomics

Table 1 Sequencing information of each BAC clone.

BAC ID	Read numbers	Read lengths(bp)	Assembled length(bp)	Estimated size (kb)	Coverage	N50 (bp)
ctfa	1020	311030	87525	90	3.46	78386
ctfb	1013	335538	103323	110	3.05	67071
ctfc	1000	324833	99742	95	3.42	51904
ctfd	996	303386	89282	90	3.37	54060
ctfe	890	279782	80143	100	2.80	41374
ctff	881	251821	75743	80	3.15	40883
ctfg	794	255225	62595	90	2.84	44454
ctfh	942	281266	85394	80	3.52	66638
ctfj	655	186358	62614	70	2.66	39606
ctfk	1095	315029	85969	100	3.15	43834
Total	9286	2844268	832330		3.14

Coverage, regarded as sequencing redundancy was calculated as the ratio of the total read length to the assembled length. N50 length is the size x such that 50% of the assembly is in units of length at least x.

ISSN: 1471-2164