Figure 1
Experimental design and hybridizations. Diagram outlining the experimental setup and hybridization strategy for microarray analysis. For each experimental group 6 pools of liver RNA extracted from 4 individuals was used. Experimental groups were: PBS control/fed liver (PFL), from fish fed a normal diet and injected with PBS: PBS control/starved liver (PSL), fish starved for 28 days and injected with PBS: Aeromonas salmonicida infected/fed liver (AFL), fish fed normal diet and injected with 100 μL of bacteria (A. salmonicida, 109 CFU ml-1); A. salmonicida infected/starved liver (ASL) fish starved for 28 days and injected with 100 μL of bacteria (A. salmonicida, 109 CFU ml-1). All fish were sampled 24 h following injection. The pooled control was an equal mixture of RNA pools from each group. All hybridizations were between experimental groups and the pooled control. For each group of 6, 12 hybridizations were performed which included a dye swap.