Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.)

Table 2 Similarity of Melampsora unisequences to sequences from UniProtKB, BasidiomycotaDB and PuccinialesDB

Library	% with homologues in UniProtKB		% with homologues in BasidiomycotaDB		% with homologues in PuccinialesDB
	NS^a	S+^b	NS	S+	NS	S+
M. larici-populina haustoria	22.6	13.9	21.5	13.9	29.6	23.6
M. larici-populina ex planta material	54.0	54.3	54.1	50.0	62.4	56.5
M. medusae f. sp. deltoidae ex planta material	56.2	50.4	54.7	45.8	64.9	56.5
M. medusae f. sp. tremuloidae ex planta material	36.7	23.6	38.3	23.2	47.4	35.4
M. occidentalis ex planta material	47.4	43.9	49.3	37.2	59.6	54.7

BasidiomycotaDB included Basidiomycota (excluding Pucciniales) EST sequence 6-frame translations and protein sequences from the non-redundant database of NCBI and gene models from Coprinus cinereus Okayama 7 (#130), Cryptococcus neoformans var. grubii serotype A, strain H99, Laccaria bicolor S238N-H82, Malassezia globosa CBS 7966, Phanerochaete chrysosporium RP78, Postia placenta MAD-698, Sporobolomyces roseus IAM 13481 and Ustilago maydis 521. PuccinialesDB included Pucciniales EST sequence 6-frame translations and protein sequences from the non-redundant database of NCBI and gene models from P. graminis f. sp. tritici CRL 75-36-700-3. BLASTX hits were considered significant when E-value ≤ 1e-4.
^aNS: Final set of unisequences not predicted to encode putative secreted proteins.
^bS+: Final set of unisequences encoding putative secreted proteins, following reassignments based on reciprocal BLAST.

ISSN: 1471-2164