Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties

Table 2 List of new mutations identified in strains NG14 and Rut-C30.

Strain	Scaffold	Probe start	Probe end	Gene ID	Element	Position	Mutation	AA change	Annotation
NG14	1	2461556	2470997	103044	IN				Unknown
NG14 and RutC30	1	2408108	2408118	103031	promoter				Unknown
NG14 and RutC30	1	2613321	2613321	53105	promoter				DNA polymerase
NG14 and RutC30	1	3525470	3525482	53811	IN exon	3525497	G→A	Ser₇₃→Leu	Clathrin adaptor complex protein
NG14 and RutC30	2	546684	546684			546703	t(4;2)(1204862;546703)
NG14 and RutC30	3	54311	54311	104067	IN not specified				Unknown
NG14 and RutC30	3	869920	870246
NG14 and RutC30	4	748146	748270			748277	t(4;22)(748277;139515)
NG14 and RutC30	4	1204838	1204852	36543	IN	1204862	t(4;2)(1204862;546703)		STE-like zinc finger-containing protein
NG14 and RutC30	10	391916	391922	78301	promoter				Cation efflux protein
NG14 and RutC30	19	385931	385937	80592	IN intron	385954-385957	CCCA→ -		Unknown
NG14 and RutC30	22	139404	139508			139476-139515	t(22;48)(139476;1667), t(4;22)(748277;139515), Δ139477-139514
NG14 and RutC30	48					1667	t(22;48)(139476;1667)
RutC-30	2	1614064	1614353	120231	promoter				G protein beta WD40 repeat
RutC-30	7	1019641	1019681	121453	IN exon				Peptidase M, zinc metallopeptidase
RutC-30	12	1014151	1014151
RutC-30	19	576820	577720	72076	IN	577175-577587	Δ577175-577587		Fungal transcription factor

See Tables 1 and 3 for description. The translocation breakpoint in the scaffold 48 does not have aCGH coordinates since the mutation site was not detected in aCGH but was identified only by LM-PCR as a breakpoint.

ISSN: 1471-2164