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Table 2 Effect of match reward and mismatch penalty on specificity and sensitivity of contiguous and discontiguous MegaBLAST searches

From: Using paired-end sequences to optimise parameters for alignment of sequence reads against related genomes

 

% total BESs

% BESs positioned

Parameters

 

&positioned&

&in tail-to-tail BACs&

&in tail-to-tail BACs&

&match reward, -r&

&mismatch penalty, -q&

Contiguous1

76.78%

32.36%

42.15%

1

-1

 

77.57%

31.91%

41.14%

2

-3

Discontiguous2

54.92%

21.94%

39.95%

1

-1

 

51.17%

22.64%

44.24%

2

-3

  1. 1word size 11, with no score filtering
  2. 2word size of 11 with a match length of 21 using a discontiguous seed, with no score filtering