Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth

BMC Genomics

Table 1 Overview of the phenotypes associated with the different prosV alleles.

Allele	Genotype	Stage of lethality	Larval taste response	Pros expression in AMC	Pros expression in CNS	Axonal routing in AMC
*V14*	Wild type (complete PGal4 remobilization)	Viable	Normal	Normal	Normal	Normal
*V13*	Partial pGal4 excision (remaining of 718 bp)	Young adult < 2 days old	Normal	Normal	altered	Normal
*V24*	Partial pGal4 excision (remaining of 7400 pb)	pupal	Intermediate	Normal	altered	Normal
V1	full length pGal4 transposon (12900 pb)	larva	Altered	absent	altered	Misrouting

(redrawn from Guenin et al. [8]).
In the prosV1 (V1) allele, the full length PGal4 transposon is inserted upstream of the pros coding region (-216 bp). prosV14 (V14) results from the correct and total remobilization of the transposon, in this strain the wild type phenotype is restored. In prosV24 (V24) and prosV13 (V13), the PGal4 element has been partially removed, respectively 7400 and 718 bp remain inserted 216 bp upstream the pros start site. The peak of developmental lethality, the taste response of late homozygous 2nd instar larva, Pros expression level in larvae and axonal misrouting are indicated for each prosV allele.
The larval taste response was measured towards 0.1 M sucrose and 0.3 M NaCl concentration that are known to respectively attract or repulse wild type Drosophila. V1 mutants were indifferent to both substances (altered taste response), V24 showed an intermediate response: they were repulsed by NaCl but remained indifferent to sucrose and V13 and V14 present a normal taste response to both substances. The Pros expression pattern is indicated by comparison to the V14 wild type: In the AMC, V1 showed no Pros expression but for the other alleles, Pros expression pattern was similar to V14. In the CNS, all mutant alleles showed a distinct altered expression pattern as compared to the wild type (further descriptions of the Pros pattern are found in the text and in [Additional file 2]).

ISSN: 1471-2164