Figure 1
From: Fragmentation of the large subunit ribosomal RNA gene in oyster mitochondrial genomes
Amplification of mtDNA and cDNA LSU rRNA gene regions in Crassostrea virginica. Diagram A presents the amplicon locations in the C. virginica and A. irradians mtDNA genome. Gel image B contains a 233 bp region of the 5'-half LSU amplified from the C. virginica genomic DNA preparation (lane 2) and cDNA preparation (lane 3), as well as a 494 bp region of the 3'-half LSU amplified from the C. virginica genomic DNA preparation (lane 4) and cDNA preparation (lane 5); lane 1 = 100 bp ladder. Gel image C: a 748 bp product (starting in the 5' half and ending in the 3' half) amplified from a continuous LSU template in Argopecten irradians genomic DNA (lane 2) and cDNA (lane 3), but was not amplified from C. virginica genomic DNA (lane 4) or cDNA (lane 5).