Table 1 Differential expression of proteins from fins by 2D-DIGE (Exp1, Exp2 and Exp3) and of their corresponding transcripts by hybridization to oligo microarrays
Spot n° | Protein Name | Accession Number | ~MW ×10-3 | ~pI | fold Exp1 | fold Exp2 | fold Exp3 | microarray |
|---|---|---|---|---|---|---|---|---|
SIGNALING | ||||||||
47 | hmgb1 | 42476233 | 16 | 7.0 | 2.4 | *3.7 | --- | 1.01 |
26 | gnb1 (G prot), βpolypep1 | 47087315 | 35 | 5.6 | 2.6 | 3.0 | --- | 1.03 |
APOPTOSIS | ||||||||
27 | Annexin A1a | 31419751 | 40 | 6.0 | 2.3 | 9.1 | --- | 1.01 |
39 | Annexin 1a | 27762256 | 44 | 6.2 | 2.5 | --- | --- | N.F. |
0 | Annexin A5b | 41107552 | 36 | 5.2 | --- | --- | -2.0 | -1.08 |
0 | Annexin 5b | 160773369 | 36 | 5.3 | --- | --- | -1.9 | N.F. |
DETOXIFICATION | ||||||||
34 | Glutathion S-Transferase | 18858197 | 16 | 8.2 | 11.0 | *2.7 | --- | 1.30 |
32 | Glutathion S-Transferase | 47086689 | 20 | 6.4 | 4.0 | *2.1 | --- | 1.30 |
HAEMATOPOIESIS | ||||||||
10 | Transferrin | 51859259 | 70 | 6.9 | 16.1 | *4.6 | --- | 2.40 |
11 | Hemopexin | 33991748 | 65 | 6.3 | 2.5 | *3.2 | --- | 5.42 |
12 | Hemopexin | 33991748 | 65 | 6.5 | 3.5 | --- | --- | 5.42 |
GLYCOLYSIS & ATP | ||||||||
24 | Aldolase fructose-bisphosphate | 37595414 | 44 | 8.3 | 5.7 | 8.2 | *1.4 | 1.15 |
45 | Triosephosphate isomerase 1b | 47271422 | 18 | 7.1 | 2.1 | *1.5 | 1.5 | 1.00 |
29 | GAPDH | 56718619 | 42 | 7.2 | 5.3 | 10.2 | *1.8 | 1.12 |
31 | GADPH | 53733367 | 40 | 8.2 | 4.2 | 8.7 | --- | 1.12 |
4 | GAPDH | 53733367 | 85 | 5.6 | -7.4 | --- | -*1.7 | 1.12 |
16 | Alpha enolase 1 | 37681795 | 57 | 6.7 | 6.0 | 17.6 | 1.8 | 1.18 |
28 | Ldhb Lactate dehydrogenase | 28277619 | 35 | 6.8 | 3.3 | --- | *2.2 | 1.04 |
33 | Carbonic anhydrase | 18858379 | 22 | 7.8 | 10.4 | --- | --- | -1.04 |
43 | Malate dehydrogenase | 47085883 | 40 | 7.8 | 2.1 | *1.7 | --- | 1.05 |
3 | ATP binding | 51571925 | 85 | 5.5 | -19.1 | --- | --- | -2.22 |
0 | ATP synthase | 41152334 | 24 | 7.0 | --- | --- | 1.5 | 1.05 |
46 | Creatine kinase | 55716037 | 15 | 6.5 | -3.4 | --- | -*1.7 | 3.16 |
20 | Creatine kinase | 55716037 | 48 | 6.4 | -3.9 | --- | -*2.0 | 3.16 |
5 | Creatine kinase | 55716037 | 90 | 5.9 | -4.0 | --- | -*2.0 | 3.16 |
CYTOSKELETON & RELATED PROTEINS | ||||||||
42 | Keratin 18 | 41351240 | 57 | 5.3 | 3.2 | 11.2 | --- | 1.08 |
38 | Keratin type I | 50370316 | 57 | 5.6 | 2.4 | --- | --- | 1.09 |
13 | Type II cytokeratin | 18858425 | 59 | 5.1 | 3.2 | 1.0 | --- | 1.23 |
50 | Tubulin, gamma assoc protein 2 | 41056243 | 85 | 6.0 | 2.4 | --- | --- | -1.01 |
30 | Skeletal alpha-actin (S. aurata) | 6653228 | 38 | 7.9 | -2.7 | -2.8 | --- | -1.53 |
21 | Alpha-tropomyosin | 18859505 | 44 | 6.6 | --- | 3.4 | 7.2 | 1.56 |
0 | Alpha-tropomyosin | 55962544 | 36 | 5.0 | --- | --- | 11.7 | 1.56 |
15 | Transgelin | 37681953 | 49 | 5.2 | -7.4 | --- | --- | 1.49 |
22 | Kinesin-like protein | 50055013 | 50 | 6.7 | -2.7 | --- | --- | -2.5 |
41 | Myosin VIa | 10116291 | 114 | 5.9 | 2.0 | *5.1 | 7.7 | 1.05 |
0 | Myosin, light chain 2 | 18859049 | 22 | 5.0 | --- | --- | 7.3 | 1.30 |
19 | CapG | 29612467 | 48 | 5.3 | 2.8 | *7.6 | --- | -1.08 |
0 | Actin capping protein | 41053959 | 34 | 5.7 | --- | --- | -1.6 | 1.09 |
6 | Scinderin | 42542770 | 80 | 6.4 | 4.1 | --- | --- | -1.14 |
7 | Scinderin | 42542770 | 80 | 6.5 | 3.8 | --- | --- | -1.14 |
17 | Cyclase-associated protein-1 | 37725381 | 58 | 8.3 | 5.0 | --- | --- | 1.66 |
35 | Dynamin 1-like | 41055508 | 5 | 5.3 | 3.0 | --- | --- | 1.01 |
48 | Semaphorin 3Gb | 57790316 | 7 | 6.4 | 3.0 | --- | --- | 1.09 |
14 | CkII protein | 39645432 | 65 | 5.3 | 2.9 | --- | --- | 1.13 |
0 | fgf20 fin regeneration | 51571925 | 26 | 6.2 | --- | --- | 1.8 | 1.26 |
0 | fgf20 fin regeneration | 51571925 | 26 | 6.9 | --- | --- | 2.2 | 1.26 |
0 | fgf20 fin regeneration | 51571925 | 26 | 7.0 | --- | --- | 2.3 | 1.26 |
0 | fgf20 fin regeneration | 51571925 | 26 | 7.2 | --- | --- | 1.8 | 1.26 |