High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

Table 1 Summary of assembly and EST data

Number of Reads	582,650
Total Bases	181 Mb
Average read length after MIRA	312
Number of contigs	75,407
Average contig length	509
Range contig length	40-3,400
Number of singletons	3,071
Number of Contigs with 2 reads	29,206
Number of Contigs with > 2 reads	43,130
Contigs with BLASTx matches (E-value ≤ 10^-6)	18,407
*Remaining contigs with additional matches (E-value ≤ 10^-2)	3,616
Contigs determined by ESTscan	17,402
**Total number of transcripts	39,425
**Total number of putatively translated amino-acids sequences	42,073

*contigs without BLASTx matches at an E-value cut-off of 10^-6 were queried again with BLASTx with an E-value cut-off of 10^-2
** The difference between the number of transcripts and total number of amino-acid sequences is due to the possibility of a contig having more than one annotated protein hit.

ISSN: 1471-2164