Figure 1
Characterization of mutant phenotypes . A: Δelp3 morphology. Wild-type (FY368) and Δelp3 (FY3851)cells were asynchronously grown at 32°C in YES. Cells were fixed and stained with DAPI. The scale bar represents 10 microns. B: Δelp3 mutants have a growth lag. Representative growth curves for wild type (triangle) (FY368) and Δelp3 (circle) (FY3851) cells in rich media (YES) at 30°C. The x-axis time scale indicates hours after inoculation that stared at a cell concentration of 4 × 105 cells/ml. The y-axis indicates number of cells/ml. C: Comparison of growth rates on YES at different temperatures and with different levels of salt. Cells were grown to exponential phase, serially diluted 5× and compared for growth on rich media after 2 or 3 days at 30°C. and 36°C, or onYES supplemented with indicated salt and grown for 2 to 4 days at 30°C. Strain list: wild-type (FY368), Δelp3(FY3851), Δmst2 (FY1890), Δgcn5 Δmst2(Hu990), Δgcn5 Δelp3 (FY3847), Δmst2 Δelp3 (FY3850), Δgcn5 Δelp3 Δmst2 (3854) D: Comparison of drug sensitivity. Exponentially growing cells were diluted 5-fold on YES plates with the indicated drugs and grown 2-3 days at 32°C. Strain list: wild-type (FY261), Δswi6 (FY1584), Δrad3 (FY1104), Δelp3 (FY 3851), Δgcn5 (FY2943), Δmst2 (FY1890), Δgcn5 Δelp3 (FY3847), Δgcn5 Δmst2(FY3361), Δmst2 Δelp3 (FY3850), Δgcn5 Δelp3 Δmst2 (3854).