Table 1 Main characteristics of the antibodies used for the experiments detailed in the manuscript.
| Â | Manufacturer | Product & Lot No. | Host species | Immunogen | Dilution used |
|---|---|---|---|---|---|
Primary antibodies | Â | Â | Â | Â | |
Neurofilament (clone N52) | Sigma | N0142 & 65K4804 | mouse | C-terminal segment of enzymatically dephosphorylated pig neurofilament 200 | 1:20,000 using immunoperoxidase staining 1:1500 using Western blotting |
Synaptophysin (clone SVP-38) | Sigma | S5768 & 90K4844 | mouse | Rat retina synaptosome | 1:5000 using immunoperoxidase staining |
Rat CD68- macrophages/monocytes (clone ED-1) | Chemicon | MAB1435 & 0606032963 | mouse | A single chain glycoprotein of 90-100 KDa that is expressed on the lysosomal membrane of myeloid cells | 1:400 using immunofluorescent staining |
Rat CD11b; RPE (clone OX-42) | Serotec | MCA275EL & 0105 | mouse | Rat peritoneal macrophages | 1:200 using immunofluorescent staining |
Glial fibrillary acid protein (clone SVP-38) | Dako | Z0334 & 00045904 | rabbit | Cow spinal cord | 1:1000 using Immunofluorescent staining |
β-actin (clone AC-15) | Sigma | A1978 & 118K4827 | mouse | Fusion of mouse myeloma cells and splenocytes immunized with a synthetic b-cytoplasmic actin N-terminal peptide | 1:5000 using Western blotting |
NeuroTrace (fluorescent Nissl stain) | Molecular Probes | N21480 & 4971-21 | - | - | 1:100 according to manufacturer's instruction |
Secondary antibodies | Â | Â | Â | Â | |
Alexa Fluor 488 | Invitrogen | A21202 & 536050 | donkey | Anti-mouse IgG (H+L) | 1:1000 using immunofluorescent staining |
Alexa Fluor 568 | Invitrogen | A10042 & 538952 | donkey | Anti-rabbit IgG (H+L) | 1:1000 using immunofluorescent staining |
Alexa Fluor 680 | Invitrogen | A21057 & 65E2-1 | goat | Anti-mouse IgG (H+L) | 1:15,000 using Western blotting |