Figure 6
PITG_18284: Alpha-carbonic anhydrase. A) Multiple sequence alignment showing the sequence similarity between PITG_18284 protein from the plastic secretome and its paralogs and well-characterized plant and human homologs. The CO2 binding residues are indicated by *. Proteins belonging to the P. infestans secretome are labeled with a signal peptide (SigP.) icon. Aligned regions are numbered in the same way in panel B to facilitate matching the sequence to the predicted protein structure. Regions indicated in blue show better conservation than regions in pink. B) Modeled protein structure of PITG_18284 with the regions shown in panel A highlighted. C) Position of PITG_18284 and other P. infestans α-CA on the FIR heat map of P. infestans (Figure 2B). D) in planta expression pattern of five P. infestans α-CAs. Non-secreted α-CAs are not induced in planta (PITG_17808 and PITG_17844), whereas secreted α-CAs show early (PITG_17842 and PITG_18284) or late induction (PITG_14412). Expression of the effector gene Avr3a is given as a reference. Dpi, days post inoculation.