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Table 1 Oak (Q. petraea and Q. robur) cDNA libraries for Sanger sequencing

From: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

Species

Library code

Library name

Library type

Kit for library construction

No. of genotypes

Tissue

Sample stage/treatment

Q. petraea

A

LG0BAC

Standard

LambdaZAP

50

bud

Quiescent buds from 2-year-old trees (Phalsbourg (57-F) and Mirecourt (88-F)) sampled in April 7th and 9th, 2004

 

B

QpBudslate

Standard

CloneMiner

2

bud

Early swelling bud sampled in March 24th and 30th , 2006 on adult trees

 

C

QpSwellingBud

Subtractive

SMART PCR, BD PCR-Select, pCR4 TOPO

20

bud

Swelling vs. quiescent buds, 1-year-old trees

 

D

QpBudquiescent

Subtractive

SMART PCR, BD PCR-Select, pCR4 TOPO

60

bud

Developing (internodes have started to grow) vs. quiescent buds, 1-year-old trees

 

E

QpVegetativeGrowth

Subtractive

SMART PCR, BD PCR-Select, pCR4 TOPO

20

bud

Quiescent vs. swelling buds, 1-year-old trees

 

F

sessile48hours

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

10

root

Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005

 

G

sessile6 hours

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

10

root

Hypoxia for 6 h. White roots from 6-month-old cuttings, 2005

 

H

Qp5stressRoots

Standard

CloneMiner

15

root

6-month-old seedlings collected in October 2006, 1/10°C 3 days, 2/35°C 4 days, 3/CO2 700 ppm, 4/water stress, 5/hypoxie 48 h

 

I

QpLeaf5stress

Standard

CloneMiner

15

leaf

6-month-old seedlings collected in October 2006 : 1/10°C 3 days, 2/35°C 4 days, 3/CO2 700 ppm, 4/water stress, 5/hypoxie 48 h

 

J

QpXyleme

Standard

Creator SMART

2

xylem

Secondary differentiating xylem sampled in May 21th, 2007 on adult trees

Q. robur

K

QrBudsEarly

Standard

CloneMiner

3

bud

Setting bud sampled in October 26th, 2006 on adult trees

 

L

QrBudslate

Standard

CloneMiner

3

bud

Swelling buds sampled in March 24th and 30th, 2007 on adult trees

 

M

LG00BAD

Standard

LambdaZAP

10

root

Fine roots under optimal fertilization and Irrigation conditions, harvested in August 2004 [48]

 

N

pedonculate6 hours

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

10

root

Hypoxia for 6 h. White roots from 6-month-old cuttings, 2005

 

O

pedonculate48 hours

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

10

root

Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005

 

P

LG0BAA

Standard

LambdaZAP

3

leaf

Young leaves sampled on adult trees in April 27th, 2004

 

Q

HighWUE

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

5

leaf

Green leaves on one-year-old cuttings, (high vs. low WUE) October 2005

 

R

LowWUE

Subtractive

SMART PCR, BD PCR-Select, pGEM-T easy

5

leaf

Green leaves on one-year-old cuttings, (low vs. high WUE) October 2005

 

S

LG0BAB

Standard

LambdaZAP

3

xylem

Secondary differentiating xylem sampled on adult trees in April 27th, 2004

 

T

QrAnoxie

Standard

Creator SMART

10

root

Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005