Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06
© Bennett et al; licensee BioMed Central Ltd. 2010
Received: 5 July 2010
Accepted: 23 November 2010
Published: 23 November 2010
The genus Neisseria contains two important yet very different pathogens, N. meningitidis and N. gonorrhoeae, in addition to non-pathogenic species, of which N. lactamica is the best characterized. Genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally.
Non-pathogenic N. lactamica exhibits very similar population structure and levels of diversity to the meningococcus, whilst gonococci are essentially recent descendents of a single clone. All three species share a common core gene set estimated to comprise around 1190 CDSs, corresponding to about 60% of the genome. However, some of the nucleotide sequence diversity within this core genome is particular to each group, indicating that cross-species recombination is rare in this shared core gene set. Other than the meningococcal cps region, which encodes the polysaccharide capsule, relatively few members of the large accessory gene pool are exclusive to one species group, and cross-species recombination within this accessory genome is frequent.
The three Neisseria species groups represent coherent biological and genetic groupings which appear to be maintained by low rates of inter-species horizontal genetic exchange within the core genome. There is extensive evidence for exchange among positively selected genes and the accessory genome and some evidence of hitch-hiking of housekeeping genes with other loci. It is not possible to define a 'pathogenome' for this group of organisms and the disease causing phenotypes are therefore likely to be complex, polygenic, and different among the various disease-associated phenotypes observed.
Comparison of the genomes of related bacteria that exhibit distinct pathogenic phenotypes can identify the genetic traits required for invasion and elucidate key steps in the evolution of virulence. The genus Neisseria, which comprises Gram negative oxidase positive diplococci that colonise the mucosa of humans and animals, provides an excellent model for this type of study as it includes species that are never or rarely pathogenic and two human pathogens of global significance, Neisseria meningitidis (the meningococcus) and Neisseria gonorrhoeae (the gonococcus) . Neisseria lactamica is closely related to the pathogenic Neisseria[2, 3] and, like them, is only ever isolated from humans; consequently, a number of studies have been undertaken to compare the non-pathogen N. lactamica with meningococci and gonococci in the hope of identifying key genetic determinants of meningococcal or gonococcal disease [4–13].
Phenotypically, the gonococcus is the most divergent of the three organisms as it colonises the urogenital tract and can be considered to be an obligate pathogen of the mucosal surface that occasionally causes disseminated infection . The meningococcus and N. lactamica are more similar in their life histories: both are obligate commensal inhabitants of the human nasopharynx that establish long-term normally asymptomatic colonisation. Carriage of N. lactamica is high in infants and young children and declines as the age of the human host population rises. The converse is true for the meningococcus, the carriage prevalence of which is low in infants and young children but rises with host age, generally reaching its highest in adolescents and young adults [15, 16]. Unlike N. lactamica, which is only anecdotally associated with invasive disease [17–19], the meningococcus can be a dangerous pathogen occasionally invading the nasal mucosa to cause septicaemia and meningitis . Although devastating for the patient, neither of these syndromes is of any benefit to the meningococcus itself as they do not normally lead to onward transmission of the bacterium, which is therefore best categorised as an 'accidental pathogen' . Nonetheless, meningococcal disease is a global phenomenon which, in some settings, occurs in large outbreaks . The idea that the colonisation of children with N. lactamica plays a role in the development of immunity to the meningococcus [23–25] has further stimulated comparative investigations of these two organisms and anti-meningococcal vaccines based N. lactamica have been proposed at various times [26, 27].
The meningococcus, gonococcus, and N. lactamica are closely related at the genetic level  and appear to have recently descended from the same ancestral population. Multilocus studies have indicated that these are maintained as separate populations by the absence or low frequency of genetic exchange among them, although rates of recombination within each of the microbiological species groups are high  and some genetic sequences are shared among species groups. The low genetic diversity observed at seven housekeeping genes of the gonococcus is consistent with this organism having evolved from a single clone that changed niche from the nasopharyngeal to the urogenital tract . N. lactamica and meningococcal populations, on the other hand, are more diverse and both populations consists of a number of clonal complexes, each comprising related genotypes [29, 30]. In the case of the meningococcus, some of these, the so-called hyperinvasive lineages, are particularly associated with invasive disease . Knowledge of the population structures of these organisms has been used to design studies of the genetic elements that are involved in virulence [4, 8, 13, 32], but to date a pathogenic genotype has yet to be determined and it seems probable that the invasive phenotype of the hyperinvasive genotypes is complex and polygenic. Here we examine the genealogical relationships among the three species, describe the determination and annotation of the first complete genome sequence of N. lactamica (isolate 020-06) and discuss the insights that this provides into the evolution of the pathogenic Neisseria.
Genealogical relationships among species
Relationships among N. meningitidis, N. lactamica and N. gonorrhoeae were investigated by an analysis of nucleotide sequences from 19 housekeeping gene loci. The loci included were those used for Neisseria MLST (abcZ, adk, aroE, fumC, gdh, pdhC, and pgm) [29, 33] supplemented with 12 additional loci (aspA, carB, dhpS, glnA, gpm, pilA, pip, ppk, pykA, rpiA, serC, talA) with alleles generated as described previously . The analysis included data from 52 N. lactamica isolates, comprising 46 unique seven locus sequence types (STs) sampled from a population of 271 isolates used to analyse diversity in N. lactamica, 20 isolates representing 20 unique STs (one ST from each clonal complex and two STs not belonging to clonal complexes) from the 107 N. meningitidis isolates originally used to define MLST , and subsequently analysed at additional loci , and seven complete Neisseria genomes downloaded from publicly available databases. These genomes include: meningococcal isolates Z2491 (serogroup A, ST-4 complex) , MC58 (serogroup B, ST-32 complex) , FAM18 (serogroup C, ST-11 complex) , 053442 (serogroup C, ST-4821 complex) , α14 , and gonococcal isolates FA1090 (The University of Oklahoma, U.S.A), and NCCP11945 . All isolates used (Additional file 1) have been deposited in the Neisseria PubMLST database: http://pubmlst.org/neisseria/, which is searchable by publication record.
Genealogies were constructed with CLONAL FRAME version 1.1 [34, 40] using 50000 burn-in iterations and 50000 Monte-Carlo Markov Chain iterations. The results from eight runs were combined to achieve maximum robustness and the 75% consensus tree was viewed using MEGA version 4.0 . To add support to the species-specific clustering, neighbour-joining trees were drawn from the same data using MEGA version 4.0 and Split Decomposition was carried out using SplitsTree version 4.10 . MEGA version 4.0 was used to determine p distances.
Determination of the complete genome sequence of N. lactamica 020-06
The genome was sequenced to an approximately 10-fold shotgun sequence, totaling 42232 end sequences, from pUC19 (with insert sizes of 1.4-2 kb; 2-2.8 kb and 3-3.3 kb respectively. 2.8-3.3 kb), and pMAQ1Sac_BstXI (with insert sizes of 5.5 kb-6; 9-10 and 10-12 kb respectively) genomic shotgun libraries using big-dye terminator chemistry on ABI3730 automated sequencers. End sequences from large insert BAC libraries in pBACe3.6_BamHI with an insert size 15-18 kb were used as a scaffold. This generated an approximate 1-fold coverage from 4670 end sequences). All repeat regions and gaps were bridged by read-pairs or end-sequenced polymerase chain reaction (PCR) products again sequenced with big dye terminator chemistry on ABI3730 capillary sequencers. The sequence was manipulated to the 'Finished' standard .
Annotation and genome comparison
An automatic gene prediction program (Glimmer3)  was used to identify coding regions. Putative orthologues were identified by reciprocal-best-match FASTA searches between N. lactamica 020-06 and meningococcal strain FAM18 amino acid sequences with cut-offs of 80% sequence length and 30% identity. The complete genome was annotated manually using the genome viewer Artemis . The N. lactamica genome sequence was compared to genomes from N. meningitidis (Z2491, MC58, FAM18, 053442 and α14) and N. gonorrhoeae (FA1090, NCCP11945) using the Artemis comparison tool (ACT) . The Dot plot figure was generated using MUMmer version 3.22  and indicates matching sequences, with codirectional and reversed regions of synteny shown in red and blue, respectively. The figure is a plot for multiple query sequences (other Neisseria) and one reference sequence (N. lactamica) where each reference/query comparison gives its dotplot. The genome sequences were aligned to start/finish at the origin of replication. The comparative analysis of the core gene dataset was carried out using an in-house pipeline of all-against-all best reciprocal FASTA searches that outputs the respective percentage (pair-wise) identity. The analysis and annotation of repeat families in N. lactamica, was carried out implementing a hidden Markov model based methodology, as previously described .
Results and Discussion
Genealogical relationships of N. lactamica, N. meningitidis, and N. gonorrheoae
The nucleotide sequence diversity for the 19 loci among the species groups as assessed by pairwise p-distances was similar, ranging from a p-distance of 0.053 between the meningococci and the gonococci to a p-distance of 0.073 between N. lactamica and N. meningitidis. The diversity within each of the groups, measured by the same means, was also similar among the N. lactamica isolates (average p-distance of 0.026) and the meningococcal isolates (average p-distance of 0.032) and appreciably lower between the two gonococcal isolates (p-distance of 0.06). These data support the conclusions reached from seven-locus multilocus sequence typing (MLST) and multilocus enzyme electrophoresis studies that the three microbiological species represent valid functional and taxonomic groups [3, 28] notwithstanding their close genetic similarity , which is consistent with recent divergence.
For the isolates included in this analysis, the number of fixed nucleotide differences greatly exceeded the number of shared polymorphisms in comparisons between the gonococci and meningococci (147 fixed differences, 33 shared polymorphisms) and the gonococci and N. lactamica isolates (316 fixed differences, 11 shared polymorphisms). This contrasted with comparisons of the meningococcal and N. lactamica isolates, where the number of fixed differences (118) was much smaller than the number of shared polymorphisms (435). Taken together these data indicate that all three species groups share an ancestral population, with the gonococcus likely to be descended from a single member of that population. N. gonorrhoeae does not exchange genetic material with other Neisseria frequently but, unlike other pathogens that have emerged by a similar process [49, 50], genetic material is regularly exchanged among gonococci such that their populations do not have a clonal structure [51, 52]. Present day meningococcal and N. lactamica populations, on the other hand, have diverged from the ancestral population without undergoing a severe bottleneck and now represent distinct species groups, with the nucleotide sequence diversity in their housekeeping genes exhibiting extensive shared ancestry.
In the current analysis there were only two cases of alleles shared among the different species groups, both at the pykA locus. The pykA-7 allele was present in 5 meningococcal isolates and one N. lactamica isolate and the pyKA-24 allele was present in one meningococcal isolate and one N. lactamica isolate. In the N. lactamica 020-06 genome this locus is immediately adjacent to an orthologue of gene NMB0088 in the meningococcal MC 58 genome, which encodes a putative immunogenic outer membrane protein orthologous to the FadL potential vaccine component of Escherichia coli. This observation complements previously published evidence for the hitch-hiking of housekeeping alleles with antigen alleles promoting genetic exchange among meningococci and N. lactamica, although such hybrids as do occasionally arise appear to be less fit and are purged by purifying selection . In conclusion, while genetic exchange between meningococci and N. lactamica has been reported for a number of genes under positive selection [55, 56], the two groups remain distinct as a result of limited genetic exchange among housekeeping genes , which is consistent with the species status of these organisms. Frequent horizontal genetic exchange among groups would prevent speciation, or if it occurs after the speciation event, would lead to a 'despeciation' process, as has been proposed recently for the gastric pathogens Campylobacter jejuni and Campylobacter coli. There is no convincing evidence of such a process occurring among the Neisseria.
As with meningococci, N. lactamica isolates are currently assigned to clonal complexes on the basis of their seven locus sequence types http://neisseria.org/nm/typing/mlst/. These clonal complexes comprise a central genotype ST and STs that share identical alleles for at least four of the seven MLST loci in the central genotype. At the time of writing, six N. lactamica clonal complexes had been identified, each named after the central genotype of the complex (the ST-595, ST-613, ST-624, ST-640, ST-1494 and ST-1540 clonal complexes), five of which were represented in the genealogical analysis reported here. Members of the same clonal complexes were consistently clustered together in the 19 locus genealogy, indicating the general robustness of the assignment of clonal complexes on the basis of seven loci. The genealogy did, however, suggest that some of the genealogical groups were larger than the currently defined clonal complexes and also indicated the existence of several other clonal complexes, currently represented by one or a few sequence types (Figure 1). The star phylogeny of N. lactamica is redolent of that of the meningococcus  and consistent with both species exhibiting a clonal complex population structure, comprising groups of related isolates that cannot be genealogically linked to each other as a consequence of high rates of intra-species horizontal genetic exchange [33, 34, 58].
On the basis of these analyses there was no a priori reason to choose a member of one particular clonal complex over any other for genome sequencing, although it was considered desirable to choose a recent bacterial isolate that had not been maintained in the laboratory for long periods of time and one that represented a common and widespread genotype. For these reasons, isolate 020-06, obtained from a six-week-old child from the UK in 1997 was chosen as the most appropriate candidate for genome sequencing. This isolate has ST-640, the central genotype of a clonal complex from which members have been isolated in the UK  and four other countries (unpublished data). The MLST profiles for these isolates can be viewed at: http://pubmlst.org/neisseria/.
Comparison of the genome sequence of N. lactamica isolate 020-06 with other Neisseria genomes
General features and gene order
General features of eight Neisseria genomes
Disease or carriage
MLST sequence type
MLST clonal complex
Genome size (bp)
G + C content (%)
Putative No. of CDSs
Gene content and core genome
The draft genomes sequences of eight human Neisseria commensal species have been compared to eleven previously sequenced Neisseria genomes  and a Neisseria core genome of 896 genes was defined, consisting mainly of housekeeping genes. This analysis, however included Neisseria elongata, which is more distantly related to the meningococcus than the other Neisseria commensals analysed, and is an unusual member of the genus in that it is rod-shaped, in contrast to other Neisseria spp. which are diplococcic. This species also shares fewer core genes with the pathogenic species than the other commensals. Further studies, involving more commensal genomes will help define further the core Neisseria genome and may also alter the species definitions of organisms currently defined as Neisseria.
For the core genome defined here CDSs ranged from 36.4% to 100% amino acid identity. However, 1180 of these CDSs had greater than 70% amino acid identity among all the genomes examined. The ten CDSs that had less than 70% amino acid identity to the corresponding CDSs in isolate Z2491 included seven that were classified as transport/membrane proteins; one unknown protein; a 3-oxoacyl-(acyl carrier protein) synthase II protein; and putative ribonuclease BN. However, the lower identity was not consistent across the genomes. For example, the transferrin-binding protein B (TbpB) in Z2491 was greater than 70% identical to the corresponding proteins in the other genomes except for the genomes of the two serogroup C meningococci (FAM18 and 053442). Whereas the TbpB in isolate 053442 was 67.3% identical to the Z2491 TbpB, the FAM18 TbpB was only 41.7% identical. Previous work has determined that there are two tbpB isotypes, with isotype 1 solely identified among N. meningitidis isolates belonging to the ST-11 clonal complex , of which isolate FAM18 is a member. The CDS encoding the porin protein PorB also showed differing degrees of identity among the genomes, with the FAM18 porin having the least identity to Z2491 (61.8%). The PorB proteins are known to be diverse  with the FAM18 porin belonging to class PorB2 and the Z2491 porin belonging to class PorB3.
The accessory genome
Adhesins/haemolysins absent from pathogenic Neisseria
% GC content
CDS immediately upstream encodes a putative haemolysin activator protein. The two genes form a two-partner secretion (TPS) pathway .
No activator protein immediately upstream.
CDS immediately upstream encodes a putative haemolysin activator protein. The two genes form a two-partner secretion (TPS) pathway .
Adjacent CDS encodes a putative TonB-dependant receptor.
Has similarity with surface fibril proteins from H. influenzae .
Immediately downstream is a putative glycosyltransferase unique to N. lactamica.
There were several genes not present in meningococci and gonococci which may have been lost by these organisms on speciation, including those encoding: a putative toxin-antitoxin system (RelEB)  and seven CDSs encoding hypothetical proteins not found in the pathogenic Neisseria upstream of these loci; a putative TonB-dependant receptor that is also present in a number of other Neisseria species (Neisseria cinerea ATCC14685, Neisseria sicca ATCC29256, Neisseria subflava NJ9703, Neisseria flavescens NRL30031, Neisseria mucosa ATCC25996 sequenced at the Genome Sequencing Center at the University of Washington and available at http://www.ncbi.nlm.nih.gov/). The N. lactamica genome appears to have two different copies of genes encoding putative L-lactate permeases. One (NLA16970), encodes an L-lactate permease similar to those found in N. gonorrhoeae and N. meningitidis and the other (NLA5610) is present only in N. lactamica 020-06 and other genomes of commensal Neisseria (N. lactamica ATCC 23970, N. cinerea ATCC14685, N. sicca ATCC29256 , N. mucosa ATCC25996, sequenced at The Genome Sequencing Center, University of Washington). Downstream of NLA5610 are genes that encode two putative TonB dependant receptors: NLA5590 which is present in pathogenic and commensal Neisseria, and NLA5600, which has only been found in N. lactamica 020-06 and commensals sequenced at The Genome Sequencing Center, University of Washington (N. lactamica N. cinerea, N. sicca, N. mucosa, N. subflava, and N. flavescens). These two genes may have been deleted from the meningococcal and gonococcal genomes, as the GC content of these sequences in N. lactamica 020-06 did not suggest horizontal genetic exchange.
Although N. lactamica is distinct from N. gonorrhoeae, there are some similarities between the two species. The most obvious is the lack of capsule in both N. lactamica and N. gonorrhoeae. In addition, the putative toxin-antitoxin system FitAB is present in N. lactamica and N. gonorrhoeae but not N. meningitidis. A gonococcal mutant that lacks fitAB grows normally extracellularly, but has an accelerated rate of intracellular replication with a concomitant increase in the rate at which this mutant traverses a monolayer of polarized epithelial cells . As N. meningitidis lacks both FitA and FitB, intracellular replication and intracellular trafficking may be greater in meningococci. NLA13470 and NLA13480 (putatively azlC and azlD respectively) are also present in N. gonorrhoeae. In N. meningitidis, azlC is truncated and azlD is not present, suggesting this region has been disrupted in N. meningitidis. A list of genes present in meningococcal genomes Z2491, MC58, FAM18, and the gonococcal genome FA1090, but absent from N. lactamica 020-06 is included in Additional file 8.
Out of a total of 134 candidate meningococcal virulence genes proposed previously, 115 (86%) have been shown to be present in the genomes of three meningococcal isolates from asymptomatic carriage (α14, capsule null; α153, serogroup 29E; and α275, serogroup W-135) . A total of 78 (58%) of these genes were present in the N. lactamica 020-06 genome (Additional file 9). The remaining 56 'N. meningitidis specific' genes were not found in all meningococcal genomes and some were present as putative pseudogenes . Functional genes found in all the meningococcal genomes analysed to date but not found in N. lactamica 020-06 included: lgtA, opcB, porA, fetB2, frpA, frpC, iga1, sodC, gna1870, natC and nlp; however, gna1870 appeared to be present in other N. lactamica isolates (our unpublished data) and many of the other genes may yet be found in N. lactamica isolates that have not been sequenced. There is an iga2 gene present in N. lactamica (NLA19200); however, this gene has a ~708 bp insertion relative to the iga2 gene in the pathogenic Neisseria and bears no resemblance to the gene that encodes the IgA protease, which is implicated in pathogenesis.
These analyses confirm that the majority of the accessory genome is shared among all three Neisseria species, probably by horizontal genetic exchange, and that a 'pathogenome' of accessory genes, which explains differences in the pathogenic phenotypes among these organisms cannot readily be defined. Some of the shared CDSs have previously been described as 'putative virulence genes' but these are better thought of as genes that confer fitness benefits to transmission only in certain circumstances or genetic backgrounds and which affect the probability of causing invasive disease only incidentally ; indeed, many of these genes have now been identified in the complete genome of N. lactamica 020-06 and other N. lactamica isolates . As meningococcal-specific sequences are thought to represent only about 5% of the genome of N. meningitidis, differences in virulence among isolates and species is likely to be due to subtle changes in their genetic organisation and in their alleles, making the determination of what constitutes a pathogenic phenotype complex for these organisms. This observation is consistent with the conclusions of a previous micro-array-based study, which suggested that a complex integrated network of genes, regulation and diversity of function in common genes could be responsible for the behavioural differences among N. lactamica, N. meningitidis and N. gonorrhoeae. Consequently, for these organisms it is not possible to attribute the emergence of an invasive phenotype to a single event, a single gene set, or loss or gain of a single function .
Comparisons of the genome of N. lactamica 020-06 with those of the meningococcus and gonococcus provide further detail as to how three closely related biological species, each with distinct genetic and phenotypic characteristics have emerged from a single ancestral population . All three have retained very similar genomes in terms of overall composition, size, and architecture, suggesting that this divergence is relatively recent. The most likely scenario for the emergence of the gonococcus remains the invasion of a single clone into a novel niche, the urogenital tract. This argument is strengthened by the presence of porA, gamma-glutamyltranspeptidase (ggt) and opc pseudogenes in N. gonorrhoeae genomes [73–75]. Speciation of N. lactamica and N. meningitidis has followed a different path, with the two populations diverging without a bottleneck event, therefore retaining substantial shared ancestral diversity. It has been proposed that the species N. meningitidis emerged at the same time that meningococcal disease was first described in 1806 [13, 76], but this is highly conjectural as commensal meningococci are likely to have been in existence for many thousands of years. It is unclear how gene flow between these groups was disrupted sufficiently for the emergence of distinct species , but perhaps this was a consequence of specialisation in the colonisation of different host age groups. There is little evidence of frequent genetic exchange among the present day populations except for genes under positive selection, mostly those encoding variable antigens [54, 56]. Occasionally housekeeping alleles adjacent to genes under positive selection are exchanged as a consequence of hitchhiking , but overall the species groups are coherent. The observed infrequent inter-species genetic exchange among housekeeping genes is supported by an estimate of the average size of an imported genetic fragment which is 1100 bp , larger than the average gene length in the meningococcal genome (852 bp) . If inter-species genetic exchange was frequent among housekeeping genes, gene replacements and therefore the sharing of alleles among species would be common. This clearly is not the case here and proving that horizontal exchange has occurred can be difficult as it requires more evidence than shared polymorphisms alone, which may have been inherited vertically from the ancestral population.
Recombination has erased any phylogenetic signal within each of these species groups at most housekeeping loci. For the single clone origin gonococcus, relatively little variation has accumulated in housekeeping genes but that which has, has been extensively reassorted. In meningococci and N. lactamica, extensive genetic diversity, much of it inherited from the common ancestral gene pool, is organised into clonal complexes, each associated with particular alleles at each of the housekeeping gene loci analysed to date. Unlike the polymorphisms, these alleles appear to post-date speciation. The core genome is very similar in terms of orthologous genes among all three species and estimates of the three species and meningococcal core genome of ~1200 and ~1300  CDSs respectively, are also very similar. This suggests that there are few differences in the core genomes of these three species and few core genes are limited to just one species group. This is also true of the accessory genome, the majority of which is shared among the three species. Genetic variation is not, however, evenly distributed among all three species, with the core genomes exhibiting distinct polymorphisms (Figure 4). Thus it appears that sequence variation in housekeeping genes, notwithstanding appreciable shared history, is particular to each species. This may be due to coherence imposed by recombination, or it may represent co-evolution within the housekeeping genes of each species group, which could help to maintain distinct groups if hybrids that contain sequence diversity of more than one group are less fit for host-to-host transmission.
There has been a consistent temptation to map an evolutionary path from the acapsulate Neisseria species, including N. lactamica and the gonococcus, via capsule null (cnl) meningococci to capsulate and therefore invasive meningococci ; however, there is no genealogical support for this view (Figure 1) and phylogenetic trees presented as providing evidence have been misinterpreted in that the position of a cnl meningococcus marginally closer to a long branch leading to N. lactamica and gonococci does not provide strong evidence for this process . It is also the case that members of characteristically cnl meningococcal clonal complexes , such as the ST-53 complex which is represented in this analysis by the complete genome of isolate α14, can acquire a capsule and cause invasive disease. Indeed, the first representative of the ST-53 complex to be isolated was a serogroup C-expressing meningococcus from a case of invasive disease . The data are, therefore, more consistent with the capsule region genes spreading though the meningococcal population post speciation by an infectious genetic exchange process, which has not reached fixation [81, 82]. Thus the cnl is the ancestral state of the meningococcal population but meningococci with the cnl do not represent ancestors of present day capsulate meningococci.
The search for a 'pathogenome' for the pathogenic Neisseria remains frustratingly, perhaps permanently, incomplete. Unlike the enteric bacteria, where transferable genetic elements that encode virulence factors ('pathogenicity associated islands', PAIs), are associated with particular strains that cause given disease syndromes , the genetics of pathogenicity in the Neisseria is more complex, with pathogenicity-associated genes distributed throughout the genome. Meningococcal population diversity is structured into clonal complexes, some of which, the hyperinvasive lineages, are more likely to cause invasive disease than others . The capsule is the primary virulence determinant, but whilst this is necessary it is not sufficient to cause disease . Other virulence determinants, such as the MDA phage  and haemoglobin receptor (HmbR)  are widely and unpredictably distributed among meningococci and non-pathogenic Neisseria, indicating the hyperinvasive phenotype is probably encoded by different genetic elements in different meningococcal lineages. If, as has been recently suggested, clonal complex structure in the meningococcus, and by extension in N. lactamica, is a consequence of the selection of genetic types that are suited to particular micro-niches , then the increased probability of invasion will be generated in different lineages by different combinations of genetic traits. If this is indeed the case, then the genetic basis of the meningococcal hyperinvasive phenotype will only become evident by the exploitation of parallel sequencing technologies  in whole-genome analyses of large collections of Neisseria isolates that are representative of diverse genotypes and phenotypes .
We acknowledge the use of core facilities at the Wellcome Trust Sanger Institute. The Wellcome Trust funded the N. lactamica 020-06 genome project. M.C.J. Maiden is a Wellcome Trust Senior Research Fellow in Basic Biomedical Science. The authors would like to thank Sonia Budroni for producing the Dot plot figure.
- Morse SA, Knapp JS: The Genus Neisseria. The Prokaryotes. Edited by: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H. 1992, New York: Springer-Verlag, 2495-2559. 2
- Guibourdenche M, Popoff MY, Riou JY: Deoxyribonucleic acid relatedness among Neisseria gonorrhoeae, N. meningitidis, N. lactamica, N. cinerea and "Neisseria polysaccharea". Annales de l'Institut Pasteur. Microbiologie. 1986, 2: 177-185. 10.1016/S0769-2609(86)80106-5.View Article
- Bennett JS, Jolley KA, Sparling PF, Saunders NJ, Hart CA, Feavers IM, Maiden MC: Species status of Neisseria gonorrhoeae: Evolutionary and epidemiological inferences from MLST. BMC Biology. 2007, 5 (1): 35-10.1186/1741-7007-5-35.PubMed CentralPubMedView Article
- Perrin A, Nassif X, Tinsley C: Identification of regions of the chromosome of Neisseria meningitidis and Neisseria gonorrhoeae which are specific to the pathogenic Neisseria species. Infect Immun. 1999, 67 (11): 6119-6129.PubMed CentralPubMed
- Perrin A, Bonacorsi S, Carbonnelle E, Talibi D, Dessen P, Nassif X, Tinsley C: Comparative genomics identifies the genetic islands that distinguish Neisseria meningitidis, the agent of cerebrospinal meningitis, from other Neisseria species. Infect Immun. 2002, 70 (12): 7063-7072. 10.1128/IAI.70.12.7063-7072.2002.PubMed CentralPubMedView Article
- Snyder LA, Davies JK, Ryan CS, Saunders NJ: Comparative overview of the genomic and genetic differences between the pathogenic Neisseria strains and species. Plasmid. 2005, 54 (3): 191-218. 10.1016/j.plasmid.2005.04.005.PubMedView Article
- Snyder LA, Saunders NJ: The majority of genes in the pathogenic Neisseria species are present in non-pathogenic Neisseria lactamica, including those designated as 'virulence genes'. BMC Genomics. 2006, 7: 128-10.1186/1471-2164-7-128.PubMed CentralPubMedView Article
- Hotopp JC, Grifantini R, Kumar N, Tzeng YL, Fouts D, Frigimelica E, Draghi M, Giuliani MM, Rappuoli R, Stephens DS, et al: Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen-specific genes. Microbiology. 2006, 152 (Pt 12): 3733-3749. 10.1099/mic.0.29261-0.View Article
- Stabler RA, Marsden GL, Witney AA, Li Y, Bentley SD, Tang CM, Hinds J: Identification of pathogen-specific genes through microarray analysis of pathogenic and commensal Neisseria species. Microbiology. 2005, 151 (Pt 9): 2907-2922. 10.1099/mic.0.28099-0.PubMedView Article
- Stabler R, Hinds J: The majority of genes in the pathogenic Neisseria species are present in non-pathogenic Neisseria lactamica, including those designated as virulence genes: response. BMC Genomics. 2006, 7: 129-10.1186/1471-2164-7-129.PubMed CentralPubMedView Article
- van Passel MW, Bart A, Luyf AC, van Kampen AH, van der Ende A: Identification of acquired DNA in Neisseria lactamica. FEMS Microbiol Lett. 2006, 262 (1): 77-84. 10.1111/j.1574-6968.2006.00366.x.PubMedView Article
- Schoen C, Joseph B, Claus H, Vogel U, Frosch M: Living in a changing environment: Insights into host adaptation in Neisseria meningitidis from comparative genomics. Int J Med Microbiol. 2007, 297 (7-8): 601-613. 10.1016/j.ijmm.2007.04.003.PubMedView Article
- Schoen C, Blom J, Claus H, Schramm-Gluck A, Brandt P, Muller T, Goesmann A, Joseph B, Konietzny S, Kurzai O, et al: Whole-genome comparison of disease and carriage strains provides insights into virulence evolution in Neisseria meningitidis. Proc Natl Acad Sci USA. 2008, 105 (9): 3473-3478. 10.1073/pnas.0800151105.PubMed CentralPubMedView Article
- Morse SA: The biology of the gonococcus. CRC Crit Rev Microbiol. 1978, 7 (2): 93-189. 10.3109/10408417909083071.PubMedView Article
- Cartwright KAV, Stuart JM, Jones DM, Noah ND: The Stonehouse survey: nasopharyngeal carriage of meningococci and Neisseria lactamica. Epidemiol Infect. 1987, 99 (3): 591-601. 10.1017/S0950268800066449.PubMed CentralPubMedView Article
- Blakebrough IS, Greenwood BM, Whittle HC, Bradley AK, Gilles HM: The epidemiology of infections due to Neisseria meningitidis and Neisseria lactamica in a northern Nigerian community. J Infect Dis. 1982, 146 (5): 626-637.PubMedView Article
- Denning DW, Gill SS: Neisseria lactamica meningitis following skull trauma. Rev Infect Dis. 1991, 13 (2): 216-218.PubMedView Article
- Orden B, Amerigo MA: Acute otitis media caused by Neisseria lactamica. European Journal of Clinical Microbiology and Infectious Disease. 1991, 10 (11): 986-987. 10.1007/BF02005462.View Article
- Lauer BA, Fisher CE: Neisseria lactamica meningitis. Am J Dis Child. 1976, 130 (2): 198-199.PubMed
- Rosenstein NE, Perkins BA, Stephens DS, Popovic T, Hughes JM: Meningococcal disease. N Engl J Med. 2001, 344 (18): 1378-1388. 10.1056/NEJM200105033441807.PubMedView Article
- Maiden MC: Dynamics of bacterial carriage and disease: lessons from the meningococcus. Adv Exp Med Biol. 2004, 549: 23-29.PubMedView Article
- Caugant DA, Maiden MC: Meningococcal carriage and disease--population biology and evolution. Vaccine. 2009, 27 (Suppl 2): B64-70. 10.1016/j.vaccine.2009.04.061.PubMed CentralPubMedView Article
- Gold R, Goldschneider I, Lepow ML, Draper TF, Randolph M: Carriage of Neisseria meningitidis and Neisseria lactamica in infants and children. J Infect Dis. 1978, 137 (2): 112-121.PubMedView Article
- Saez-Nieto JA, Dominguez JR, Monton JL, Cristobal P, Fenoll A, Vazquez J, Casal J, Taracena B: Carriage of Neisseria meningitidis and Neisseria lactamica in a school population during an epidemic period in Spain. J Hyg. 1985, 94 (3): 279-288. 10.1017/S0022172400061507.PubMed CentralPubMedView Article
- Olsen SF, Djurhuus B, Rasmussen K, Joensen HD, Larsen SO, Zoffman H, Lind I: Pharyngeal carriage of Neisseria meningitidis and Neisseria lactamica in households with infants within areas with high and low incidences of meningococcal disease. Epidemiol Infect. 1991, 106: 445-457. 10.1017/S0950268800067492.PubMed CentralPubMedView Article
- Gorringe A, Halliwell D, Matheson M, Reddin K, Finney M, Hudson M: The development of a meningococcal disease vaccine based on Neisseria lactamica outer membrane vesicles. Vaccine. 2005, 23 (17-18): 2210-2213. 10.1016/j.vaccine.2005.01.055.PubMedView Article
- Kim JJ, Mandrell RE, Griffiss JM: Neisseria lactamica and Neisseria meningitidis share lipooligosaccharide epitopes but lack common capsular and class 1, 2, and 3 protein epitopes. Infect Immun. 1989, 57 (2): 602-608.PubMed CentralPubMed
- Vázquez JA, de la Fuente L, Berron S, O'Rourke M, Smith NH, Zhou J, Spratt BG: Ecological separation and genetic isolation of Neisseria gonorrheae and Neisseria meningitidis. Curr Biol. 1993, 3 (9): 567-572. 10.1016/0960-9822(93)90001-5.PubMedView Article
- Maiden MCJ, Bygraves JA, Feil E, Morelli G, Russell JE, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant DA, et al: Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci USA. 1998, 95 (6): 3140-3145. 10.1073/pnas.95.6.3140.PubMed CentralPubMedView Article
- Bennett JS, Griffiths DT, McCarthy ND, Sleeman KL, Jolley KA, Crook DW, Maiden MC: Genetic diversity and carriage dynamics of Neisseria lactamica in infants. Infect Immun. 2005, 73 (4): 2424-2432. 10.1128/IAI.73.4.2424-2432.2005.PubMed CentralPubMedView Article
- Yazdankhah SP, Kriz P, Tzanakaki G, Kremastinou J, Kalmusova J, Musilek M, Alvestad T, Jolley KA, Wilson DJ, McCarthy ND, et al: Distribution of serogroups and genotypes among disease-associated and carried isolates of Neisseria meningitidis from the Czech Republic, Greece, and Norway. J Clin Microbiol. 2004, 42 (11): 5146-5153. 10.1128/JCM.42.11.5146-5153.2004.PubMed CentralPubMedView Article
- Bille E, Zahar JR, Perrin A, Morelle S, Kriz P, Jolley KA, Maiden MC, Dervin C, Nassif X, Tinsley CR: A chromosomally integrated bacteriophage in invasive meningococci. J Exp Med. 2005, 201 (12): 1905-1913. 10.1084/jem.20050112.PubMed CentralPubMedView Article
- Holmes EC, Urwin R, Maiden MCJ: The influence of recombination on the population structure and evolution of the human pathogen Neisseria meningitidis. Mol Biol Evol. 1999, 16 (6): 741-749.PubMedView Article
- Didelot X, Urwin R, Maiden MC, Falush D: Genealogical typing of Neisseria meningitidis. Microbiology. 2009, 155 (10): 3176-3186. 10.1099/mic.0.031534-0.PubMed CentralPubMedView Article
- Parkhill J, Achtman M, James KD, Bentley SD, Churcher C, Klee SR, Morelli G, Basham D, Brown D, Chillingworth T, et al: Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature. 2000, 404 (6777): 502-506. 10.1038/35006655.PubMedView Article
- Tettelin H, Saunders NJ, Heidelberg J, Jeffries AC, Nelson KE, Eisen JA, Ketchum KA, Hood DW, Peden JF, Dodson RJ, et al: Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science. 2000, 287 (5459): 1809-1815. 10.1126/science.287.5459.1809.PubMedView Article
- Bentley SD, Vernikos GS, Snyder LA, Churcher C, Arrowsmith C, Chillingworth T, Cronin A, Davis PH, Holroyd NE, Jagels K, et al: Meningococcal Genetic Variation Mechanisms Viewed through Comparative Analysis of Serogroup C Strain FAM18. PLoS Genet. 2007, 3 (2): e23-10.1371/journal.pgen.0030023.PubMed CentralPubMedView Article
- Peng J, Yang L, Yang F, Yang J, Yan Y, Nie H, Zhang X, Xiong Z, Jiang Y, Cheng F, et al: Characterization of ST-4821 complex, a unique Neisseria meningitidis clone. Genomics. 2008, 91 (1): 78-87. 10.1016/j.ygeno.2007.10.004.PubMedView Article
- Chung GT, Yoo JS, Oh HB, Lee YS, Cha SH, Kim SJ, Yoo CK: Complete genome sequence of Neisseria gonorrhoeae NCCP11945. J Bacteriol. 2008, 190 (17): 6035-6036. 10.1128/JB.00566-08.PubMed CentralPubMedView Article
- Didelot X, Falush D: Inference of bacterial microevolution using multilocus sequence data. Genetics. 2007, 175 (3): 1251-1266. 10.1534/genetics.106.063305.PubMed CentralPubMedView Article
- Kumar S, Nei M, Dudley J, Tamura K: MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 2008, 9 (4): 299-306. 10.1093/bib/bbn017.PubMed CentralPubMedView Article
- Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23 (2): 254-267. 10.1093/molbev/msj030.PubMedView Article
- Chain PS, Grafham DV, Fulton RS, Fitzgerald MG, Hostetler J, Muzny D, Ali J, Birren B, Bruce DC, Buhay C, et al: Genomics. Genome project standards in a new era of sequencing. Science. 2009, 326 (5950): 236-237. 10.1126/science.1180614.PubMedView Article
- Delcher AL, Bratke KA, Powers EC, Salzberg SL: Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics. 2007, 23 (6): 673-679. 10.1093/bioinformatics/btm009.PubMed CentralPubMedView Article
- Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B: Artemis: sequence visualization and annotation. Bioinformatics. 2000, 16 (10): 944-945. 10.1093/bioinformatics/16.10.944.PubMedView Article
- Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J: ACT: the Artemis Comparison Tool. Bioinformatics. 2005, 21 (16): 3422-3423. 10.1093/bioinformatics/bti553.PubMedView Article
- Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL: Versatile and open software for comparing large genomes. Genome Biol. 2004, 5 (2): R12-10.1186/gb-2004-5-2-r12.PubMed CentralPubMedView Article
- Fermer C, Kristiansen B-E, Sköld O, Swedberg G: Sulphonamide resistance in Neisseria meningitidis as defined by site-directed mutagenesis could have its origin in other species. J Bacteriol. 1995, 177: 4669-4675.PubMed CentralPubMed
- Achtman M: Evolution, population structure, and phylogeography of genetically monomorphic bacterial pathogens. Annu Rev Microbiol. 2008, 62: 53-70. 10.1146/annurev.micro.62.081307.162832.PubMedView Article
- Keim PS, Wagner DM: Humans and evolutionary and ecological forces shaped the phylogeography of recently emerged diseases. Nat Rev Microbiol. 2009, 7 (11): 813-821. 10.1038/nrmicro2219.PubMed CentralPubMedView Article
- O'Rourke M, Stevens E: Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J Gen Microbiol. 1993, 139: 2603-2611.PubMedView Article
- O'Rourke M, Spratt BG: Further evidence for the non-clonal population structure of Neisseria gonorrhoeae: extensive genetic diversity within isolates of the same electrophoretic type. Microbiology. 1994, 140: 1285-1290. 10.1099/00221287-140-6-1285.PubMedView Article
- Sardiñas G, Yero D, Climent Y, Caballero E, Cobas K, Niebla O: Neisseria meningitidis antigen NMB0088: sequence variability, protein topology and vaccine potential. J Med Microbiol. 2009, 58 (Pt 2): 196-208. 10.1099/jmm.0.004820-0.PubMedView Article
- Linz B, Schenker M, Zhu P, Achtman M: Frequent interspecific genetic exchange between commensal neisseriae and Neisseria meningitidis. Mol Microbiol. 2000, 36 (5): 1049-1058. 10.1046/j.1365-2958.2000.01932.x.PubMedView Article
- Zhu P, van der Ende A, Falush D, Brieske N, Morelli G, Linz B, Popovic T, Schuurman IG, Adegbola RA, Zurth K, et al: Fit genotypes and escape variants of subgroup III Neisseria meningitidis during three pandemics of epidemic meningitis. Proc Natl Acad Sci USA. 2001, 98 (9): 5234-5239. 10.1073/pnas.061386098.PubMed CentralPubMedView Article
- Bennett JS, Thompson EA, Kriz P, Jolley KA, Maiden MC: A common gene pool for the Neisseria FetA antigen. Int J Med Microbiol. 2009, 299 (2): 133-139. 10.1016/j.ijmm.2008.06.010.PubMed CentralPubMedView Article
- Sheppard SK, McCarthy ND, Falush D, Maiden MC: Convergence of Campylobacter species: implications for bacterial evolution. Science. 2008, 320 (5873): 237-239. 10.1126/science.1155532.PubMedView Article
- Maynard Smith J, Smith NH, O'Rourke M, Spratt BG: How clonal are bacteria?. Proc Natl Acad Sci USA. 1993, 90 (10): 4384-4388. 10.1073/pnas.90.10.4384.View Article
- Jolley KA, Chan MS, Maiden MC: mlstdbNet - distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics. 2004, 5 (1): 86-10.1186/1471-2105-5-86.PubMed CentralPubMedView Article
- Treangen TJ, Ambur OH, Tonjum T, Rocha EP: The impact of the neisserial DNA uptake sequences on genome evolution and stability. Genome Biol. 2008, 9 (3): R60-10.1186/gb-2008-9-3-r60.PubMed CentralPubMedView Article
- van Passel MW, van der Ende A, Bart A: Plasmid diversity in Neisseriae. Infect Immun. 2006, 74 (8): 4892-4899. 10.1128/IAI.02087-05.PubMed CentralPubMedView Article
- Bentley SD, Parkhill J: Comparative genomic structure of prokaryotes. Annu Rev Genet. 2004, 38: 771-792. 10.1146/annurev.genet.38.072902.094318.PubMedView Article
- Vogel U, Morelli G, Zurth K, Claus H, Kriener E, Achtman M, Frosch M: Necessity of molecular techniques to distinguish between Neisseria meningitidis strains isolated from patients with meningococcal disease and from their healthy contacts. J Clin Microbiol. 1998, 36 (9): 2465-2470.PubMed CentralPubMed
- Darling AE, Miklos I, Ragan MA: Dynamics of genome rearrangement in bacterial populations. PLoS Genet. 2008, 4 (7): e1000128-10.1371/journal.pgen.1000128.PubMed CentralPubMedView Article
- Schoen C, Tettelin H, Parkhill J, Frosch M: Genome flexibility in Neisseria meningitidis. Vaccine. 2009, 27 (Suppl 2): B103-111. 10.1016/j.vaccine.2009.04.064.PubMed CentralPubMedView Article
- Marri PR, Paniscus M, Weyand NJ, Rendon MA, Calton CM, Hernandez DR, Higashi DL, Sodergren E, Weinstock GM, Rounsley SD, et al: Genome sequencing reveals widespread virulence gene exchange among human Neisseria species. PLoS One. 2010, 5 (7): e11835-10.1371/journal.pone.0011835.PubMed CentralPubMedView Article
- Harrison OB, Maiden MC, Rokbi B: Distribution of transferrin binding protein B gene (tbpB) variants among Neisseria species. BMC Microbiology. 2008, 8 (1): 66-10.1186/1471-2180-8-66.PubMed CentralPubMedView Article
- Derrick JP, Urwin R, Suker J, Feavers IM, Maiden MCJ: Structural and evolutionary inference from molecular variation in Neisseria porins. Infect Immun. 1999, 67 (5): 2406-2413.PubMed CentralPubMed
- Serino L, Virji M: Genetic and functional analysis of the phosphorylcholine moiety of commensal Neisseria lipopolysaccharide. Mol Microbiol. 2002, 43 (2): 437-448. 10.1046/j.1365-2958.2002.02755.x.PubMedView Article
- Magnuson RD: Hypothetical functions of toxin-antitoxin systems. J Bacteriol. 2007, 189 (17): 6089-6092. 10.1128/JB.00958-07.PubMed CentralPubMedView Article
- Mattison K, Wilbur JS, So M, Brennan RG: Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. J Biol Chem. 2006, 281 (49): 37942-37951. 10.1074/jbc.M605198200.PubMedView Article
- Nassif X:Genomics of Neisseria meningitidis. Int J Med Microbiol. 2002, 291 (6-7): 419-423. 10.1078/1438-4221-00148.PubMedView Article
- Feavers IM, Maiden MCJ: A gonococcal porA pseudogene: implications for understanding the evolution and pathogenicity of Neisseria gonorrhoeae. Mol Microbiol. 1998, 30: 647-656. 10.1046/j.1365-2958.1998.01101.x.PubMedView Article
- Takahashi H, Watanabe H: A gonococcal homologue of meningococcal gamma-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent. BMC Microbiol. 2005, 5: 56-10.1186/1471-2180-5-56.PubMed CentralPubMedView Article
- Zhu P, Morelli G, Achtman M: The opcA and (psi)opcB regions in Neisseria: genes, pseudogenes, deletions, insertion elements and DNA islands. Mol Microbiol. 1999, 33 (3): 635-650. 10.1046/j.1365-2958.1999.01514.x.PubMedView Article
- Vieusseux G: Memoire sur le maladie qui a régné a Genêve au printemps de 1805. Journal de Médecine, Chirurgie et Pharmacie. 1806, II: 163-165.
- Fraser C, Hanage WP, Spratt BG: Recombination and the nature of bacterial speciation. Science. 2007, 315 (5811): 476-480. 10.1126/science.1127573.PubMed CentralPubMedView Article
- Jolley KA, Wilson DJ, Kriz P, McVean G, Maiden MC: The influence of mutation, recombination, population history, and selection on patterns of genetic diversity in Neisseria meningitidis. Mol Biol Evol. 2005, 22 (3): 562-569. 10.1093/molbev/msi041.PubMedView Article
- Weber MVR, Claus H, Maiden MCJ, Frosch M, Vogel U: Genetic mechanisms for loss of encapsulation in polysialyltransferase-gene-positive meningococci isolated from healthy carriers. Int J Med Microbiol. 2006, 296 (7): 475-484. 10.1016/j.ijmm.2006.05.004.PubMedView Article
- Feavers IM, Gray SJ, Urwin R, Russell JE, Bygraves JA, Kaczmarski EB, Maiden MCJ: Multilocus sequence typing and antigen gene sequencing in the investigation of a meningococcal disease outbreak. J Clin Microbiol. 1999, 37 (12): 3883-3887.PubMed CentralPubMed
- Levin BR: Periodic selection, infectious gene exchange and the genetic structure of E. coli populations. Genetics. 1981, 99 (1): 1-23.PubMed CentralPubMed
- Dolan-Livengood JM, Miller YK, Martin LE, Urwin R, Stephens DS: Genetic basis for nongroupable Neisseria meningitidis. J Infect Dis. 2003, 187 (10): 1616-1628. 10.1086/374740.PubMedView Article
- Hacker J, Blum-Oehler G, Muhldorfer I, Tschape H: Pathogenicity Islands of virulent bacteria: structure, function and impact on microbial evolution. Mol Microbiol. 1997, 23 (6): 1089-1097. 10.1046/j.1365-2958.1997.3101672.x.PubMedView Article
- Stephens DS: Biology and pathogenesis of the evolutionarily successful, obligate human bacterium Neisseria meningitidis. Vaccine. 2009, 27 (Suppl 2): B71-77. 10.1016/j.vaccine.2009.04.070.PubMed CentralPubMedView Article
- Harrison OB, Evans NJ, Blair JM, Grimes HS, Tinsley CR, Nassif X, Kriz P, Ure R, Gray SJ, Derrick JP, et al: Epidemiological evidence for the role of the hemoglobin receptor, HmbR, in meningococcal virulence. The Journal of Infectious Diseases. 2009, 200 (1): 94-98. 10.1086/599377.PubMed CentralPubMedView Article
- Buckee CO, Jolley K, Recker M, Penman B, Kriz P, Gupta S, Maiden MC: Role of selection in the emergence of lineages and the evolution of virulence in Neisseria meningitidis. Proc Natl Acad Sci USA. 2008, 105 (39): 15082-15087. 10.1073/pnas.0712019105.PubMed CentralPubMedView Article
- Medini D, Serruto D, Parkhill J, Relman DA, Donati C, Moxon R, Falkow S, Rappuoli R: Microbiology in the post-genomic era. Nat Rev Microbiol. 2008, 6 (6): 419-430.PubMed
- Maiden MC: Population genomics: diversity and virulence in the Neisseria. Curr Opin Microbiol. 2008, 11 (5): 1-5. 10.1016/j.mib.2008.09.002.View Article
- van Ulsen P, Rutten L, Feller M, Tommassen J, van der Ende A: Two-partner secretion systems of Neisseria meningitidis associated with invasive clonal complexes. Infect Immun. 2008, 76 (10): 4649-4658. 10.1128/IAI.00393-08.PubMed CentralPubMedView Article
- St Geme JW, Cutter D, Barenkamp SJ: Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J Bacteriol. 1996, 178 (21): 6281-6287.PubMed CentralPubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.