Figure 6

Northern blot analysis to detect csRNAs in total RNA from streptococci. (A) Detection of csRNAs in S. mitis B6. Northern blot analysis of total RNA isolated from S. mitis B6. RNAs were detected by hybridization of digoxigenin-labeled probes complementary to individual csRNAs as indicated at the bottom of the blot. (B) Detection of csRNAs in S. oralis Uo5. Northern blot analysis of total RNA isolated from S. mitis B6. RNAs were detected by hybridization of digoxigenin-labeled probes complementary to individual csRNAs as indicated at the bottom of the blot. (C) Detection of csRNAs in S . sanguinis SK36. Northern blot analysis of total RNA isolated from S. mitis B6. RNAs were detected by hybridization of digoxigenin-labeled probes complementary to individual csRNAs as indicated at the bottom of the blot. (D) Detection of csRNA9 from plasmid pST0 cloned in S. pneumoniae lacking endogenous csRNAs. Northern blot analysis of total RNA isolated from S. mitis B6. RNAs were detected by hybridization of digoxigenin-labeled probes complementary to individual csRNAs as indicated at the bottom of the blot. (E) Dependence of csRNA expression on CiaR in S. oralis Uo5. Northern blot analysis of total RNA isolated from S. oralis Uo5 wild type and a ciaR::aad9 mutant derivative. The blot was hybridized with digoxigenin-labeled probes complementary to all csRNAs. Lane 1: S. oralis Uo5 RNA. Lane 2: S. oralis ciaR::aad9 RNA. Lane 3: In vitro synthesized RNA size standard.