Figure 6

Quantitative real time PCR verification of selected genes. Microarray expression profiles for Cyp2d4, Por, Dbp and Arntl were assessed by qRTPCR using gene specific Taqman probes. Housekeeping gene beta-actin was used to standardize expression. In each case, the qRTPCR data very closely approximated the microarray expression levels and are temporally correlated in both sexes. Pearson's correlations were calculated between averaged microarray (uA) and qRTPCR (RT) data for each gene and coefficients ranged from R = 0.810 to 0.985. Blue and red lines indicate male and female qRTPCR data, respectively (n = 5), and black lines represent microarray data (n = 5, error bars represent SEM for qRTPCR).