Skip to main content

Table 3 Mapping statistics for sea bass BAC-ES comparatively mapped to the stickleback chromosomes VIII to XIV.

From: The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

A

stickleback chromosome

CHR VIII

CHR IX

CHR X

CHR XI

CHR XII

CHR XIII

CHR XIV

 

stickleback chromosome size [bp]

19,368,704

20,249,479

15,657,440

16,706,052

18,401,067

20,083,130

15,246,461

 

genetic linkage group sea bass

LG4

LG7

LG9

LG8

?

LG20

LG19

B

sea bass BAC-contigs

30 (44)

37 (45)

31 (37)

30 (27)

27 (35)

26 (38)

18 (33)

 

largest BAC-contig [bp]

4,711,769 (3,129,353)

3,058,355 (1,487,429)

1,368,409 (1,368,348)

4,014,839 (3,993,770)

4,710,925 (2,950,196)

3,478,171 (3,182,188)

3,545,956 (1,757,446)

 

% of chr. covered by BAC-contigs

85.8% (77.0%)

82.9% (69.7%)

84.2% (75.4%)

83.3% (74.4%)

88.5% (78.1%)

90.6% (81.8%)

88.0% (77.5%)

 

number of BACs in min. tiling path

188 (177)

195 (174)

152 (140)

155 (147)

177 (160)

194 (176)

150 (142)

 

paired end aligned consistent BACs

869 (517)

855 (448)

712 (410)

816 (494)

902 (559)

1,121 (678)

792 (414)

 

one end aligned BACs

1,002 (1,190)

1,190 (1,168)

897 (879)

1,101 (1,089)

1,211 (1,364)

1,194 (1,266)

931 (1,017)

C

inconsistently aligned same chr.

110 (112)

84 (90)

90 (118)

81 (86)

58 (90)

42 (80)

59 (62)

 

potential intra-chr. rearrangements between stickleback and sea bass

10

12

16

13

12

9

6

 

intra-chr. rearrangements also found between medaka and stickleback

5

9

13

7

9

3

2

 

BAC-contigs with same neighbour in medaka as in stickleback

15

12

6

8

6

7

9

D

total annotated genes in ensembl

876

1,009

802

1,050

1,000

970

738

 

% genes covered by BAC-contigs

83.4% (75.9%)

80.0% (70.5%)

81.2% (70.7%)

83.5% (75.3%)

86.8% (76.5%)

89.6% (81.6%)

85.2% (74.9%)

  1. Rows (A) show a summary of the 21 reference chromosomes (stickleback assembly: BROAD S1, Feb 2006) and the known corresponding genetic linkage groups of sea bass according to Chistiakov et al. [30]. Rows (B) summarize consistently mapped BAC-ES, BAC-contigs and reference genome/chromosome coverage of the minimal tiling path. Rows (C) focus on inconsistently mapped BAC-ES, which where further analysed for potential intra-chromosomal rearrangements and compared to the medaka genome as a second reference genome. Rows (D) display the number of annotated genes in stickleback and the percentage of them covered by the sea bass BAC-map. Values in brackets "()" show results for a comparative map that was built by using only BAC-ES data, while all other values represent results of improved mapping using BAC-ES together with whole genome shotgun data.