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Table 4 Mapping statistics for sea bass BAC-ES comparatively mapped to the stickleback chromosomes XV to XXI.

From: The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

A

stickleback chromosome

CHR XV

CHR XVI

CHRXVII

CHRXVIII

CHRXIX

CHR XX

CHR XXI

 

stickleback chromosome size [bp]

16,198,764

18,115,788

14,603,141

16,282,716

20,240,660

19,732,071

11,717,487

 

genetic linkage group sea bass

LG13

LG15

LG1?

LG17

LG6

LG16/18?

LG18?

B

sea bass BAC-contigs

20 (29)

24 (38)

30 (30)

35 (43)

38 (43)

22 (35)

9 (17)

 

largest BAC-contig [bp]

3,426,120 (1,723,928)

6,616,511 (2,444,746)

3,516,936 (2,019,946)

1,934,982 (1,280,086)

2,450,075 (2,125,025)

7,220,725 (2,296,377)

4,139,228 (2,305,187)

 

% of chr. covered by BAC-contigs

89.3% (82.5%)

88.4% (79.0%)

88.5% (78.7%)

83.6% (70.6%)

86.6% (78.2%)

90.3% (82.8%)

87.4% (83.3%)

 

number of BACs in min. tiling path

156 (150)

182 (167)

163 (138)

170 (143)

206 (185)

194 (178)

111 (116)

 

paired end aligned consistent BACs

969 (619)

916 (533)

784 (459)

783 (373)

1,004 (628)

1,077 (639)

639 (393)

 

one end aligned BACs

1,017 (1,057)

1,163 (1,162)

1,110 (1,145)

989 (1,040)

1,204 (1,187)

1,178 (1,183)

693 (691)

C

inconsistently aligned same chr.

73 (96)

47 (74)

37 (34)

68 (78)

104 (128)

30 (46)

7 (10)

 

potential intra-chr. rearrangements between stickleback and sea bass

10

8

6

12

14

8

2

 

intra-chr. rearrangements also found between medaka and stickleback

9

4

4

6

8

6

2

 

BAC-contigs with same neighbour in medaka as in stickleback

3

7

4

9

7

3

4

D

total annotated genes in ensembl

779

799

698

761

1,037

927

460

 

% genes covered by BAC-contigs

87.9% (84.1%)

88.5% (80.4%)

85.5% (77.4%)

82.7% (69.5%)

82.5% (75.5%)

89.5% (85.1%)

93.9% (86.7%)

  1. Rows (A) show a summary of the 21 reference chromosomes (stickleback assembly: BROAD S1, Feb 2006) and the known corresponding genetic linkage groups of sea bass according to Chistiakov et al. [30]. Rows (B) summarize consistently mapped BAC-ES, BAC-contigs and reference genome/chromosome coverage of the minimal tiling path. Rows (C) focus on inconsistently mapped BAC-ES, which where further analysed for potential intra-chromosomal rearrangements and compared to the medaka genome as a second reference genome. Rows (D) display the number of annotated genes in stickleback and the percentage of them covered by the sea bass BAC-map. Values in brackets "()" show results for a comparative map that was built by using only BAC-ES data, while all other values represent results of improved mapping using BAC-ES together with whole genome shotgun data.