Figure 3

Tissue-specific alternative splicing of TPM1 and TPM2 are confirmed using asMIPs. Tissue-specific isoforms and splice-score data are color-coded; skeletal muscle is black, stomach is blue, and reference sample data is yellow. (A & B) Schematic representations of two muscle specific isoforms. Exons are represented by numbered boxes. Thin lines depict introns. (C & D) Graphs of the raw sequence counts for asMIPs interrogating the exon junctions specified (e.g. 4-5, 5-6 et cetera). Signal variation for constitutive junctions is due predominantly to sequence-based probe effects. (E & F) Graphs of the M-scores for each junction in which probe effects, gene expression differences and experimental variance have all been accounted for. The first five junctions listed for the gene are constitutively spliced and the remaining junctions are alternatively spliced. In (E & F) both the sequencing data (squares) and array data (diamonds) are presented for comparison. The gray dashed lines show the cutoff (+/-1.3) used to make splicing calls. The tissue-specific splicing of these control genes are clear; known constitutive junctions map near zero, alternatively spliced junctions all map above or below the cutoffs.