A molecular inversion probe assay for detecting alternative splicing

BMC Genomics

Table 1 asMIP targets

Gene	tissue specificity	con¹	alt²	total
CAMK2D*	neural	8 (4)	5 (5)	13 (9)
CLTB*	neural	3 (3)	3 (3)	6 (6)
EHBP1*	neural	8 (3)	4 (4)	12 (7)
ERC1*	neural	8 (4)	10 (10)	18 (14)
FEZ2*	neural	2 (2)	9 (9)	11 (11)
MARK4	neural	8	3	11
MINK1	neural	11	10	21
MLLT4	neural	9	10	19
MYH10	neural	15	8	23
MYO6	neural	10	6	16
TPD52	neural	3	6	9
TPM1	muscle	5	7	12
TPM2	muscle	5	6	11
TPM3	muscle	4	6	10
ACTB	control	5	0	5
GAPDH	control	8	0	8
TUBA1B	control	3	0	3
	totals:	115	93	208

Numbers in parentheses correspond to total junctions analyzed by qPCR.
¹ The number of predicted constitutive exon junctions targeted by asMIPs indentified from RefSeq [21].
² The number of potentially alternatively spliced exon junctions targeted identified from RefSeq [21], of which 58 were confirmed in the literature to be alternatively spliced in the tissues we studied [10, 20].
* Genes that were assayed with both asMIPs and qPCR.

ISSN: 1471-2164