Figure 5
From: New methods for next generation sequencing based microRNA expression profiling
qPCR confirmation of sequencing data. MicroRNA expression levels for both 5p and 3p transcripts from single precursor transcripts in HH16 whole chicken embryo and heart tube. 5 s rRNA was used as an internal control to normalize gene expression. Left and right column represent miSeq and qPCR derived expression levels, respectively. The bars represent mean expression levels ± sd. * indicates a significant difference in gene expression relative to whole embryo.