Figure 1

Point mutation discovery in an evolved yeast genome. SNPs that are supported by a read depth of ≥6 and ≥5 in the evolved and ancestor strain sequence data, respectively, are shown. These SNPS are also supported by ≥80% of the base calls for the position in the evolved strain and ≥70% of the calls in the parental strain. Tiling array-discovered SNPs in DBY11331 are labeled green. Sanger-sequence traces from evolved and ancestor genomes validate the SNP at chr11: 207,469 in the adaptive clone. Sanger sequence data derived from a population sample suggest that the chr11: 207,469 SNP is in high frequency in the evolving population time-point from which the evolved clone was isolated (bottom trace).