Figure 5

Integration of metabolomic and transcriptomic data using pathway mapping. Overlaid ¹H-NMR spectra from day 14 samples showing a dose dependent decrease in glycogen. A similar response is seen at all other time points (A). The glycogen synthesis pathway demonstrating the correlation between transcriptional and metabolic changes. Hexokinase D, which is involved in control of the rate of glycogen synthesis, is decreased indicating that the decrease in glycogen is due to decreased synthesis (B). Glutathione synthesis pathway. Due to the extraction procedure, all glutathione detected is oxidized [38]. The increase in glutathione at days 1, 3 and 7 is likely to be due to an increase in glutamate cysteine ligase expression (C). The key to the gene expression data is given in (D).