Figure 5

Expression analysis of class I Clp ATPase genes in different parts of rice plant based on the microarrays performed for rice. A. Expression pattern of OsClpC and OsClpD transcripts in different parts of rice. B. Expression pattern of OsClpC and OsClpD transcripts in different developmental stages of rice. In silico analysis shown in A and B was performed using the Genevestigator database. The analysis included data from a total of 166 array experiments, and included 28 different tissues, organs or growth stages. C. Expression analysis of rice Clp ATPase transcripts in response to cold, heat and oxidative stresses. Hierarchical cluster display of expression profile for rice ClpC and ClpD genes showing differential expression in rice (color bar represents the log2 expression values; green color representing low level expression, yellow shows medium level expression and red signifies the high level expression). D. Semi-quantitative RT-PCR of rice ClpC and ClpD genes under different stress conditions. The following stress conditions were used; HS: heat shock treatment (45°C; 10', 20', 30' and 1 h). LT: low temperature stress (8 ± 2°C; 24 h and 48 h). SS: NaCl stress (150 mM; 3 h and 6 h). DS: PEG treatment (12% PEG8000; 3 h and 6 h). PCR was performed for 25 cycles and products were resolved on 1% agarose gel.