Trichomonas vaginalis vast BspA-like gene family: evidence for functional diversity from structural organisation and transcriptomics

Table 2 Features of the 18 TvBspA proteins encoded by scaffold DS113361

Locus tag^a	Align^b	Protein length	LRR^c start	LRR^c end	Pa/Pr^c	E-value^d	TMD^e position	Notable features^f
Subfamily #22 (14 entries)
TVAG_133050	110	837	15	796	1	2.0E-69		Conserved C-terminus
Subfamily #200
TVAG_133260	649	285	56	273	0	3.0E-21
TVAG_133330	650	666	23	656	0	7.0E-43
TVAG_133300	651	566	65	517	1	4.0E-46	468-490, 539-561
TVAG_133380	652	174	38	173	0	9.0E-16
TVAG_133420	653	392	66	390	0	3.0E-29		SP
TVAG_133230	678	205	3	201	1	9.0E-26
TVAG_133270	679	205	3	201	1	9.0E-26
Subfamily #228
TVAG_133390	680	638	3	555	2	4.0E-43
TVAG_133240	681	601	5	358	0	3.0E-26	513-535	Conserved CT
TVAG_133280	682	583	5	352	0	7.0E-26	495-517	Conserved CT
TVAG_133310	683	595	4	382	1	4.0E-30	505-527	Conserved CT
TVAG_133340	684	448	3	210	1	3.0E-17	358-380	Conserved CT
TVAG_133430	685	734	3	474	1	2.0E-35	744-666	Conserved CT
Subfamily #275
TVAG_133290	689	318	3	227	2	8.0E-34
TVAG_133410	690	562	1	426	1	8.0E-40
TVAG_133360	691	333	2	263	1	5.0E-26
TVAG_133220	692	363	51	362	0	2.0E-31

^aThe locus tag numbers give an indication of the proximity of the different TvBspA encoding genes on the scaffold, with an incremental of 10 for each neighbouring genes (see additional file 8, Figure S3). The numbers of entries for each of the four proteins subfamilies identified by CLUSS2 (additional file 1, Table S1) are indicated.
^bAlignment position in the output file generated by ClustalW. The position of the 18 TvBspA proteins in the global TvBspA alignment (additional file 1, Table S1) is indicated.
^cThe position of TpLRR inferred by BlastP (see Methods section). The numbers indicate the start and end of the TpLRR. The entries positive for either the TpLRR profile or pattern (Pa/Pr), or both, are indicated by a 1 and 2 respectively.
^dE-values for the PSI-Blast search (2^nd iteration) that followed a PHI-Blast (see Methods section).
^eEntries with inferred transmembrane domains (TMD) are indicated with the shown start and end position of the TMD. All such entries had the N-termini with the TpLRR domain inferred to be "external" by two or more TMD predictors (see Methods section).
^fTVAG_133420 has an inferred signal peptide (SP). The five entries from subfamily #228 with a TMD all share a similar cytoplasmic tail - see Figure 3.

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