Trichomonas vaginalis vast BspA-like gene family: evidence for functional diversity from structural organisation and transcriptomics

Table 4 Microarray data for 13 TvBspA with significant modulation in their mRNA concentration upon exposure to different iron concentration

Locus tag	Annotation^a	Subfamily membership^{a, b}	EST^a	Modul.^c	p-value	qRT-PCR^d
High iron culture condition
TVAG_397210	BspA-like	#43, 10 members^e	1	2.2	1.5 × 10-4	2.16 ± 0.002
TVAG_441420	BspA-like, SP, TMD	#374, singleton, divergent TpLRR	1	1.55	8.4 × 10-3	2.02 ± 0.04
TVAG_191490	BspA-like, SP, PG, GRD	#168, 12 members	1	1.29	5.5 × 10-3	---
TVAG_396970	BspA-like	#44, 15 members*	2	1.23	4.8 × 10-3	---
TVAG_080240	BspA-like	#44, 15 members*	6	1.18	8.5 × 10-3	---
Low iron culture condition
TVAG_093850	BspA-like	#184, 3 members	2	-1.27	2.9 × 10-3	---
TVAG_299910	BspA-like	#69, 3 members *	16	-1.25	6.8 × 10-3	---
TVAG_493590	BspA-like	#384, 6 members	4	-1.24	3.7 × 10-3	---
TVAG_129450	BspA-like, PG	#30, 15 members	1	-1.18	2.4 × 10-5	---
TVAG_530030	BspA-like	#99, 2 members	7	-1.15	2.5 × 10-3	-2.06 ± 0.02
TVAG_341990	BspA-like	#215, 3 members	1	-1.14	3.7 × 10-3	---
TVAG_176920	BspA-like, TMD	#341, singleton, divergent TpLRR	1	-1.13	7.8 × 10-3	---
TVAG_235070	BspA-like	#73, Singleton	1	-1.12	3.9 × 10-4	---
Control genes
TVAG_238830	Hydrogenosomal malic enzyme subunit B	---	---	1.67	1.5 × 10-4	2.51 ± 0.01
TVAG_165030	Cytosolic malate dehydrogenase	---	---	-1.33	2.8 × 10-4	-1.62 ± 0.03

^aSee additional file 1, Table S1 for full details. SP, signal peptide; TMD, transmembrane domain; PG, pseudogene; GRD, glycine-rich domain.
^bSF, subfamilies identified with CLUSS2. *Related sequences identified by visual inspection of the alignment.
^cPositive values indicate up-regulated genes and negative values down-regulated genes. All values are derived from quadruplets with indicated mean value (Log scale) and significance (p-values) of modulation.
^dqRT-PCR was performed in quadruplets with indicated mean and standard deviations.

ISSN: 1471-2164