Volume 11 Supplement 4
Construction of a genetic AND gate under a new standard for assembly of genetic parts
© Ayukawa et al; licensee BioMed Central Ltd. 2010
Published: 2 December 2010
Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.
We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.
We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
The development of synthetic biology promotes the construction of artificial genetic systems with desired functions inside living cells [1, 2]. The bottleneck in the realization of a functioning system is the appropriate regulation of gene expression . Thus far, many studies in synthetic biology have seen difficulties in regulating all genes in the system to work collaboratively. In order to construct an efficient biosynthetic pathway of a precursor anti-malaria drug, the regulation of several gene expressions inside Saccharomyces cerevisiae had to be modified in addition to the introduction of exogenous genes . This and other studies using similar modifications indicate that well-designed gene regulation is important to constructing a well-functioning genetic system [5, 6].
The engineering of promoters is an effective method for the fine regulation of gene expression for realization of the desired function. Most promoters contain a -35/-10 sequence where an RNA polymerase binds. Also, some promoters contain regulatory protein-binding sites called operators. By changing the strength of the -35/-10 sequence and the operator sequence arrangement, the promoter for the appropriate regulation of gene expression can be constructed.
Regulation assemblies are composed of many types of DNA units. Some of the DNA units are small pieces of DNA containing a protein-binding site. Assembling these DNA units allows for preparation of a synthetic promoter library from which genetic logic gates have been isolated . Another type of DNA unit is a large fragment of DNA containing a protein coding sequence. By assembling several DNA units containing promoter sequences and DNA units containing coding sequences of regulatory proteins, a genetic toggle switch and a repressilator were constructed inside Escherichia coli[8, 9].
Rational design is one approach to establishing a regulation assembly of DNA units. For example, a synthetic promoter integrating input of arabinose and Isopropyl β-D-1-thiogalactopyranoside (IPTG) was designed by allocation of operators for two different regulatory proteins, AraC and LacI . With a different design, Anderson et al. constructed an AND gate based on an interaction between nonsense suppressor tRNA and mRNA with an internal stop codon . For construction of each variation of these assemblies, variation-specific DNA construction was required.
BioBricks were developed as standard DNA parts for construction of designed biological systems [12–16]. For construction of genetic circuits, BioBricks include several classes of genetic parts: ribosome-binding site, protein-coding sequence, protein-binding site, and -35/-10 sequence. Furthermore, the BioBricks parts can be combined laterally because of the standardization of parts, which are standardized by introduction of two sets of restriction sites. The BioBricks standard allows repeating attachments of new parts to the foreside and backside of the already connected parts. This standardization, however, does not allow insertion of parts between already connected parts even though such an insertion is important for modification of a prototype genetic assembly.
In this study, by modifying BioBricks standardization, we developed a new standardization that allows for insertion of parts into an established assembly of other parts and constructed a new genetic multi-input regulation assembly. In addition to the sets of restriction sites in BioBricks, we used another set of restriction sites for the new standardized parts assembly. The new standardization allows for insertion of parts between two pre-connected parts. In fact, by introducing operator parts into the regulatory region of a plasmid that already had allocated another species of an operator part, we constructed a new promoter integrating the inputs of two different inducers into a transcription output. The new assembly of operator parts can also be called a genetic AND gate.
Design of a genetic AND gate
Construction of a standardized platform of the regulatory region
Construction of a gate regulated by Lacl repressors
Construction of a gate regulated by a LuxR activator
By inserting an operator part containing a LuxR-binding site, we constructed another plasmid named PLuxR-GFP, which generates GFP output dependent on the input of N(β-ketocaproyl)-DL-homoserine lactone (AHL). The LuxR operator part is composed of a LuxR expression cassette, a LuxR operator sequence and a weak -35/ -10 sequence called luxpR, which exist in the natural lux operon . In the presence of AHL, a LuxR protein binds to the LuxR operator and activates the transcription.
Construction of a genetic AND gate by assembly of operator parts
In order to modify the regulatory region of a gene, we here developed a new standard by modifying the BioBricks standard and constructed a new genetic AND gate from standardized biological parts. The Lux-Lac AND gate we constructed is composed of LuxR and LacI operators, whose bindings to the regulatory proteins are controlled by AHL and IPTG, respectively. In order to realize the desired function, our AND gate has two important properties. One is that we prepared standardized parts, each of which has one operator site, and combined them by modular assembly. In contrast, another method of assembly in the synthetic Ara-lac AND gate  requires a unique DNA with multiple operator sites for each designed combination of parts. Another important property is that our method is based on the simple mechanism of gene regulation. It would be difficult to simultaneously use several complicated regulation assemblies such as a nonsense-suppression dependent system .
Our modification of BioBricks standard allows insertion of parts into an established assembly of other parts. Although In-Fusion BioBrick assembly reported very recently  may allow similar insertion of parts with further modifications of their method, it requires primers, each of which covers two parts next to each other in the design of an assembly. This combination-dependent synthesis reduces the utility of the established compatibility of each BioBricks part.
In contrast to design-based strategy we examined in this study, other researchers have used a combinatorial strategy to construct artificial biological devices. In order to establish the fine regulation of a genetic circuit, Guet et al. constructed a combinatorial library in which repressor coding sequence and promoters were connected in various orders on a plasmid . By using another combinatorial strategy, Cox III et al. constructed several AND gates and OR gates . In their study, operators for LacI, TetR, AraC, or LuxR were randomly inserted to the regulatory region. Though several gates were isolated, several nonfunctional gates were constructed at the same time. The design-based strategy is more effective for creating assemblies since it does not require huge resources. A hybrid strategy in which a combinatorial strategy and our strategy are combined would be effective to implement an artificial biological device with specific quantitative parameter dependence on inputs. In the combinatorial library, a regulatory protein should correspond to multiple operator parts, each of which has a different binding property for the protein.
The number of transcriptional factors and operators used in synthetic biology is increasing, and a combinatorial explosion in the number of possible assemblies must occur in the future. As a method to combat this combinatorial explosion, the design strategy assisted by computer simulations and mathematical models will be effective. Even though the wet-based method has middle-sized parallelism due to the number of cells screened, as indicated by the studies of DNA computer, it would consume a lot of molecules to solve a problem stemming from a combinatorial explosion. The parallelism in computer-based evaluation of assemblies is indeed much lower than that in wet-based evaluation, but the computer-based evaluation time for each assembly will be much shorter in the future. In addition, the activity predicted from computer design often could not correctly reflect the activity of a wet molecule in the present situation. In the future, however, technological advances will reduce the difference between the predicted value and the real value. Thus, computer-assisted rational design will become more effective than the combinatorial strategy.
The genetic logic gates established with our method or its modified version would make the cells recognize the pattern of multiple input-molecules. These engineered cells can be applied in drug-delivery systems, bioremediations, and material-synthesizing systems. A cell that recognizes a lot of inputs replies well to each situation. For example, engineered cells can be designed to release appropriate medicine inside patients upon recognizing tumour markers. Also, the cells that recognize the concentration of pollutants in the environment can be designed to absorb and degrade them. Cells that recognize the coexistence of key intermediates in a metabolic pathway can be designed to optimize the material-synthesizing system. To realize of such a technology, we must prepare a database of regulatory proteins each of which binds both of an input-molecule and an operator. Perhaps the Registry of Standard Biological Parts, which becomes larger and larger every year, is a good candidate database. The Registry of Standard Biological Parts would be more effective than other databases, such as Regulon DB, since the inventory of physical BioBricks parts and information in the database are linked together.
We implemented a new standard to assemble multiple operator parts in the regulatory region by expanding the BioBricks standard. By assembling LacI and LuxR operator parts with this standardization, we constructed a new genetic AND gate. The easy assembly of multiple operator parts promotes modification of the regulation of gene expression, which is important for reprogramming cellular behavior.
Construction of plasmids
Plasmid pSB-GFP was constructed by introducing Sal I, BamH I, Bgl II, and Mlu I restriction sites to BBa_I7100, which was obtained from the Registry of Standard Biological Parts . In order to introduce these sites, PCR mutagenesis was used with the following primers: BglMlu_RBS_plus (5'-GGAGATCTGTCGGATAACGCGTGAGATTAAAGAGGAGAAATACTAGATG-3') and BamSal_pSB3_minus (5 '-GAC AGGATCC AGTCGTC AGTCGACCTCT AGAAGCGGCCGCG-3').
The LacI operator part and Placq were produced by ligation of phosphorylated and annealed oligonucleotides with T4 DNA ligase. The following oligonucleotides were used:
LacI operator part: (5'-GATCGGAATTGTGAGCGGATAACAATTCCAGATCTA TCGTAA-3') and (5'-CGCGTTACGATAGATCTGGAATTGTTATCCGCTCACAATTCC-3');
Placq:(5'-TCGACCGTGACGGATCCTGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCC-3') and (5'-GATCGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACC AGGATCCGTC ACGG-3').
In order to construct Placq-GFP, a pair of phosphorylated and annealed Placq oligonucleotides was ligated to the pSB-GFP digested with Sal I and Bgl II. A LuxR operator part was produced by digesting BBF2621, which was also obtained from the Registry of Standard Biological Parts . In order to construct PLuxR-GFP, we prepared a PLuxR fragment containing LuxR expression cassette, a LuxR operator sequence, and luxpR by digesting BBa_F2621 with Eco R I and Spe I. The fragment was ligated to the pSB-GFP, which was digested with Eco R I and Xba I. In order to construct Placq-LacI-GFP, Placq-Lacltandem-GFP, and PLuxR-LacItandem-GFP, each pair of the phosphorylated and annealed oligonucleotides was ligated to its parent plasmid digested with Bgl II and Mlu I.
Construction of E.coli strain DH5αLacI
The E. coli strain DH5αLacI was obtained by transforming DH5α with plasmid pTrc99A [GenBank: U13872], which expresses the LacI repressor.
In order to quantitatively determine the performance of the operator parts in the reporter plasmid, the change of fluorescence intensities of the reporter strains in the presence and the absence of the inducer were measured. Overnight cultures of reporter strains grown at 37 °C in LB medium containing appropriate antibiotics were diluted 1:100 in the medium and were incubated at 37 °C as fresh cultures. After their OD600 reached 0.6, the fresh cultures were diluted 1:1 in the medium in the presence or absence of various inducers: 1 mM IPTG and/or 4.5 nM N(β-ketocaproyl)-DL-homoserine lactone. After 8-hour incubation at 37 °C, 0.15 ml of each culture was moved to a 96-well plate and its raw fluorescence intensity was measured with a fluorometer (FLA5000, FUJI). The fluorescence intensity was calculated by dividing the measured raw fluorescence intensity by the culture volume and the OD600 value. A strain containing the Placq-GFP plasmid, which constitutively expresses GFP, was used as a positive control. A strain containing the pSB-GFP plasmid, which does not express GFP, was used as a negative control.
Enzymes, media, and chemicals
Restriction enzymes and polynucleotide kinase were purchased from Takara (Shiga, Japan). Pyrobest DNA polymerase for PCR mutagenesis was purchased from Takara (Shiga, Japan). T4 DNA ligase was purchased from Nippon Gene (Tokyo, Japan). DNA sequencing reactions were performed using the BD sequencing kit purchased from Applied Biosystems (CA, USA). All reactions were performed according to the manufacturer's instructions. Synthetic oligonucleotides and sequencing primers were purchased either from Operon (Tokyo, Japan) or Sigma-Aldrich-Japan (Tokyo, Japan). Antibiotics were added to the growth media at the following concentrations: 50 μg/ml ampicillin and 30 μg/ml kanamycin. IPTG and standard chemicals, all of PA grade, were purchased from Nacalai (Kyoto, Japan), and N(β-ketocaproyl)-DL-homoserine lactone was obtained from Sigma-Aldrich Japan.
List of abbreviations
- E. :
coli Escherichia coli
This research has been partially supported by the PRESTO program of JST for DK and KAKENHI programs from JSPS and MEXT (219649 for SA, 14085203 for MY and DK, and 21650018 for MH and DK).
This article has been published as part of BMC Genomics Volume 11 Supplement 4, 2010: Ninth International Conference on Bioinformatics (InCoB2010): Computational Biology. The full contents of the supplement are available online at http://www.biomedcentral.com/1471-2164/11?issue=S4.
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