Figure 5
Single-cell tracking identifies different profiles of transcriptional activation. 293T cells transfected with p(GRE)x3-AdMLP-VC5 were induced with 1 × 10-7M dexamethasone and tracked over 24 hours. (A, C) Tracks of cells inducing (A) and remaining unchanged (C) over 24 hours. Each cell track is represented by a line where each dot represents a different timepoint and the color describes the time of induction from 0 (blue) to 24 hours (red). The lower teal line represents the mean of the starting population, while the upper teal line shows the mean + 3 standard deviations. Single-cell time-lapse images are shown for cells that have similar starting ECFP expression levels, but whose response to dexamethasone is either high (B) or remains unchanged over time (D).