Figure 3
From: Applicability of RNA standards for evaluating RT-qPCR assays and platforms
Precision of real-time PCR platforms. Within-run qPCR precision across a range of transcript abundance levels (copies per ng total RNA) are displayed for cDNA or pre-amplified cDNA quantified using ABI 7900HT system or BioMark. Mean variation (SD) of Ct values ± SD is displayed based on data from eight different RNA standards from three independent qPCR experiments.