Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Table 3 Real time RT-PCR expression analysis of selected melon genes.

ID	Homology	E-value	Annotation	ISPaVe strain/dpi	I/R	Fold-change (Infect. vs. mock)	SD
P200	MU48195	0	ACC oxidase	1070/4 dpi	I	7.04	2.80
P557	MU51460	3E-45	Respiratory burst oxidase homolog protein C	1070/4 dpi	I	61.92	14.73
P1100	MU54228	2E-44	Protein translocase SEC61	1018/2 dpi	R	1.87	0.37
P1370	MU50486	1E-112	calcium-dependent protein kinase CDPK1444	1070/4 dpi	I	3.81	0.66
P554	MU45886	2E-27	13S-lipoxygenase	1018/21 dpi	I	1.26	0.14
P767	MU47701	4E-50	Endochitinase 1 precursor	1018/21 dpi	I	473.05	146.45
P769	MU43951	6E-61	UBQ14 (ubiquitin 14)	1018/21 dpi	I	25.34	24.29

Seven melon transcripts, differentially expressed following Fusarium oxysporum f. sp. melonis (FOM) infection according to cDNA-AFLP experiments, were selected for validation by real time RT-PCR. Each gene was validated at the same time point and with the same FOM strain that generated a differential cDNA-AFLP profile. The FOM strains are ISPaVe170 race1 and ISPaVe1018 race 1,2. Results are shown as fold change values in infected samples in comparison to mock-inoculated controls and are the average of three technical replicates. I/R: induced or repressed according to cDNA-AFLP experiments. SD: standard deviation.

ISSN: 1471-2164