Figure 6
From: Discovery of barley miRNAs through deep sequencing of short reads
Northern blot detection of three novel miRNAs, GPB125, GPB235 and GPB1131, from barley leaf tissues. U6 was used as loading control. Sizes of each miRNA and U6 are indicated. Uppercase P and GP represent the Pallas cultivar and the Golden Promise cultivar, respectively. GP also indicates Golden Promise plants grown under well-watered conditions. P (-p) represents Pallas plants grown in soil supplied with 22.5 mg phosphate (KH2PO4)/kg dry soil, while P (+p) represents Pallas plants grown in soil supplied with 75 mg phosphate (KH2PO4)/kg dry soil. GP (DREB3) represents Golden Promise plants transformed with the wheat DREB3 gene. GP (-water) represents Golden Promise plants grown under drought conditions (see Methods for details).