Table 1 Stage characterization, timing, and functional enrichment throughout

Unfertilized egg

1

0

Kinase

0.9950

0.0187

0.0187

1 cell

2

1.5 ± 0.25

2 cells

3

2.5 ± 0.20

ATP

0.9991

0.0065

0.0065

Fatty Acid

0.9981

0.0122

0.0122

4 cells

4

3.0 ± 0.44

Transcription Factor

0.0309

0.9953

0.0660

8 cells

5

5 ± 0.51

16 cells

6

6 ± 0.50

32 cells

7

7.5 ± 0.50

Pentose Pathway

0.9954

0.0476

0.0476

Early Morula

8

8.5 ± 0.51

Translation

0.9919

0.0254

0.0254

Late Morula

9

9.5 ± 0.51

Hatching

1

0.0017

0.0017

Ubiquitination

0.9973

0.0207

0.0207

Early Blastula

10

10 ± 0.70

Calcium Oxidative

0.9958

0.04832

0.0432

Phosphorylation

0.9969

0.0114

0.0114

Post Translational

0.9994

0.0047

0.0047

Signalling

0.9974

0.0154

0.0154

Flat Blastula

11

12 ± 1.32

Ribosomal

0.0002

1

0.0002

Transcription Factor

0.0034

0.9994

0.0061

Pre-early Gastrula

12

15 ± 1.83

Early Gastrula

13

19 ± 1.63

Blastoderm

14

21 ± 1.25

Pre-mid Gastrula

15

25 ± 2.38

DEAD

0.9999

0.0046

0.0046

Pre-mid Gastrula

16

28.5 ± 2.24

Fatty Acid

0.9976

0.0060

0.0077

Kinase

0.0065

0.9984

0.0112

Oxidative

Phosphorylation

0.9790

0.0425

0.0633

Protease

0.9976

0.0058

0.0067

Ribosomal

1

< .0001

< .0001

Translation

0.9980

0.0049

0.0077

Mid-Gastrula

17

31 ± 1.75

Late Gastrula

18

34 ± 1.75

Transcription Factor

0.9986

0.0067

0.0067

Early Neurula

19

38 ± 2.51

Glycolysis

0.9993

0.0038

0.0038

Ribosomal

0.9987

0.0042

0.0042

Late Neurula

20

42 ± 3.42

Kinase

0.9954

0.0237

0.0237

3-4 Somites

21

44 ± 4.67

Transcription Factor

0.9895

0.0475

0.0475

6-9 Somites

22

49 ± 4.42

Glutathione

0.9974

0.0318

0.0318

Oxygen

0.9988

0.0115

0.0115

Ribosomal

0.9900

0.0100

0.0100

Transcription Factor

0.0231

1

0.0535

Heart Formation

23

54 ± 5.39

ATP

0.9978

0.0134

0.0134

Ribosomal

0.9995

0.002

0.002

Heart Beat Initiation

24

65 ± 5.32

Protease

0.9994

0.0028

0.0028

Starch

0.9969

0.0359

0.0359

Transcription Factor

0.9907

0.0249

0.0381

Onset of Circulation

25

72 ± 5.22

ATP

0.9991

0.0055

0.0055

Oxygen

1

< .0001

< .0001

Growth and Organo-differentiation

26

80 ± 5.71

27

90 ± 7.80

28

102 ± 11.35

Transcription Factor

0.9860

0.0430

0.0430

29

110 ± 13.31

Inositol Signalling

Pathway

0.9969

0.0237

0.0237

Post-translational

0.9999

0.0009

0.0009

Ribosomal

0.0183

0.9974

0.0312

30

120 ± 13.31

31

140 ± 12.06

Ribosomal

0.0405

1

0.0076

Steroid

0.9996

0.0052

0.0052

Ubiqutination

0.9916

0.0468

0.0468

32

160 ± 11.72

Pentose Pathway

0.9989

0.0194

0.0194

33

180 ± 10.31

Channels

0.9975

0.0152

0.0152

Ribosomal

0.0044

1

0.0108

RNA

0.9921

0.0248

0.0248

34

195 ± 9.46

Fatty Acid

0.0462

1

0.0754

Pre-hatching

35

212 ± 12.80

Ribosomal

0.0215

0.9951

0.0503

Ribosomal

< .0001

1

< .0001

RNA

0.0463

0.9854

0.0859

Starch

0.9996

0.0036

0.0036

Hatching

36

226 ± 11.25

DEATH

0.9978

0.0280

0.0280

Growth

37

238 ± 10.01

ATP

0.9929

0.0285

0.0285

38

256 ± 9.95

Structural

1

< .0001

< .0001

Superoxide

Dismutase

0.9999

0.0044

0.0044

39

256 ± 9.95

40

290-374

Ribosomal

0.0186

1

0.0342

± 30.29

Structural

0.9965

0.0109

0.0109