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Table 4 Enzymes involved in fatty acid biosynthesis and metabolism identified by annotation of the D. tertiolecta transcriptome

From: Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Enzyme

Symbol

EC Number

Number of transcripts

1%Sequence alignment with corresponding enzymes in model organisms (Accession #)

    

C. reinhardtii

V. carteri

Fatty acid biosynthesis

     

Biotin carboxylase

BC

6.3.4.14

3

2NM

46 (EFJ41621.1)

Acetyl-CoA carboxylase

ACC

6.4.1.2

7

28 (XP_001700442.1)

28 (EFJ46223.1)

malonyl-CoA-ACP transacylase

MAT

2.3.1.39

4

NM

62 (EFJ49352.1)

Beta-ketoacyl-ACP synthase I

KAS I

2.3.1.41

7

NM

NM

Beta-ketoacyl-ACP synthase II

KAS II

2.3.1.179

12

NM

NM

Beta-ketoacyl-ACP synthase III

KAS III

2.3.1.180

1

NM

NM

Beta-ketoacyl-ACP reductase

KAR

1.1.1.100

7

63 (XP_001703473.1)

61 (EFJ52014.1)

3R-hydroxyacyl-ACP dehydrase

HAD

4.2.1.-

2

NM

NM

Enoyl-ACP reductase (NADH)

EAR

1.3.1.9

5

NM

NM

Oleoyl-ACP thioesterase

OAT

3.1.2.14

1

NM

NM

Acyl-ACP thioesterase A

FatA

3.1.2.14, 3.1.2.-

1

NM

NM

Acyl-ACP thioesterase B

FatB

3.1.2.14, 3.1.2.-

0

NM

NM

Fatty acid desaturation

     

Δ9 Acyl-ACP desaturase

AAD

1.14.19.2

5

76 (XP_001691597.1)

76 (EFJ49192.1)

Δ12(ω6)-Desaturase

Δ12D

1.14.19.6

3

54 (XP_001691669.1), 47 XP_001693068.1)

54 (XP_002955859.1), 47 (XP_002949932.1)

Δ15(ω3)-Desaturase

Δ15-D

1.14.19.-

2

NM

NM

Fatty acid elongation

     

3-Hydroxyacyl-CoA dehydrogenase

CHAD

1.1.1.35

9

NM

NM

Long-chain-3-hydroxyacyl-CoA dehydrogenase

LCHAD

1.1.1.211

2

NM

NM

Enoyl-CoA hydratase

ECH

4.2.1.17

16

NM

70 (EFJ49010.1)

Trans-2-enoyl-CoA reductase (NADPH)

TER

1.3.1.38

3

90 (XP_001690095.1)

NM

Palmitoyl-CoA hydrolase

PCH

3.1.2.22

1

19 (XP_001698944.1)

19 (EFJ40725.1)

Fatty acid catabolism

     

Long-chain acyl-CoA synthetase

ACSL

6.2.1.3

3

77 (XP_001693692.1)

77 (EFJ51208.1)

Acyl-CoA oxidase

AOx

1.3.3.6

2

39 (XP_001699193.1)

NM

Acyl-CoA dehydrogenase

ACADM

1.3.99.3

16

45 (XP_001699193.1), 84 (XP_001693484.1), 73 (XP_001695945.1)

NM

Enoyl-CoA hydratase

ECH

4.2.1.17

16

NM

70 (EFJ49010.1)

Long-chain 3-hydroxyacyl-CoA dehydrogenase

LCHAD

1.1.1.211

2

NM

NM

Acetyl-CoA acyltransferase

ACAT

2.3.1.16

1

NM

NM

3-hydroxyacyl-CoA dehydrogenase

CHAD

1.1.1.35

9

NM

NM

Acetyl-CoA C-acetyltransferase

thiL

2.3.1.9

6

39 (XP_001694888.1)

34 (EFJ47048.1), 34 (EFJ39622.1), 34 (EFJ46961.1)

3-hydroxybutyryl-CoA epimerase

 

5.1.2.3

8

NM

NM

Enoyl-CoA isomerase

ECI

5.3.3.8

8

NM

NM

Alcohol dehydrogenase

ADH

1.1.1.1

3

80 (XP_001693934.1)

53 (EFJ45245.1)

Aldehyde dehydrogenase (NAD+)

ALDH

1.2.1.3

3

24 (XP_001696928.1)

21 (EFJ40853.1)

Ferredoxin-NAD+ reductase

FNR

1.18.1.3

1

NM

NM

  1. 1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.
  2. 2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.
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BMC Genomics

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