Figure 6

PPARγ/C/EBPα overlap increases retention of binding sites. (A) Bar diagrams illustrating the fraction of C/EBPα only, PPARγ only and overlapping PPARγ-C/EBPα binding sites (x axis) in mouse mouse that are either lost (gray), or found as a C/EBPα only (blue), PPARγ only (red) or PPARγ-C/EBPα (purple) binding site in human adipocytes. Numbers above the bars correspond to the total number of mouse sites in each group. (B) Retention of mouse C/EBPα and PPARγ binding sites in mouse adipocytes (x axis) in the corresponding human regions. The bars represent fractions of mouse binding sites retained as C/EBPα-only (blue), PPARγ-only (red), and overlap (purple) binding sites in human. The x axis breaks up each set of transcription factor binding sites according to, whether it is C/EBPα- or PPARγ-only in mouse, or whether the site is also overlapped with the other factor in mouse.*P = 3.5*10^-3 (C/EBPα overlap vs. C/EBPα only (Fisher Exact Test)); #P = 6.2*10^-4 (PPARγ overlap vs. PPARγ only (Fisher Exact Test)). (C) Violin plots (see Fig. 3B for explanation) showing the distribution of tag enrichment in orthologous human regions, broken up depending on whether a site is overlapped or not in mouse adipocytes as in Fig. 6B. *P = 0.007 (C/EBPα overlap vs. C/EBPα only (Wilcoxon test)); #P = 3.9*10^-4 (PPARγ overlap vs. PPARγ only (Wilcoxon test)) (D) Retention of C/EBPα-only or PPARγ-only and PPARγ-C/EBPα binding sites in mouse, broken up by expression clusters. *P = 8.6*10^-15 (clusters 3+4 vs. all other clusters (Fisher Exact Test)); #P = 0.01 (cluster 4 vs. all other clusters (Fisher Exact Test)). (E) Violin plots showing the distribution of tag enrichment in orthologous human regions, broken up depending on whether a site is overlapped or not and on associated gene cluster in mouse adipocytes. #P = 8.072*10^-3 (cluster 4 vs. All clusters (Wilcoxon test)); *P = 0.001 (cluster 4 vs. all other clusters (Wilcoxon test)).