Gene discovery in EST sequences from the wheat leaf rust fungus Puccinia triticina sexual spores, asexual spores and haustoria, compared to other rust and corn smut fungi

Table 3 Stage-specific Pt unigene counts and representation in Pgt

stage	# unique unigenes ^a	predicted fungal ^b		# matching Pgt homologs ^d
		counts	normalized ^c
ptp	961	911	2246	563 (62%)
ptt	801	697	2259	374 (54%)
pte	100	88	818	47 (54%)
ptu	486	369	1131	152 (41%)
ptg	3308	811	811	475 (59%)
ptih	1283	973	2430	292 (30%)

^a number of non-redundant sequences obtained from the indicated specific developmental stage only: ptp, pycniospores; ptt, teliospores; pte, ae ciospores; ptu, dormant urediniospores; ptg, germinated urediniospores; ptih, isolated haustoria
^b number of unigenes predicted to be fungal
^c number of predicted fungal unigenes for that stage was normalized using quality reads in relation to the stage with the highest sampling, ptg (bold type)
^d BLASTX search of Pgt genome-predicted proteins at e < 10^-5 (percent of actual predicted fungal unigenes)

ISSN: 1471-2164