Figure 3

Expression profiling of miR-3099 novel miRNA in mouse embryonic stem cells (with conditional allele for Dicer), E3.5 blastocysts, E7 and E9.5 embryos. (A) Stemloop RT-qPCR analysis of miR-3099 novel miRNA in mouse embryonic stem (mES) cells with conditional allele for Dicer1. mES cells with and without Dicer1 activity are denoted by Dicer c/- and Dicer -/-, respectively (n = 3 per group). (B) Expression of miR-3099 in E3.5 blastocysts (n = 14; pooled) and the E7 whole embryo (n = 3). (C-F) Whole mount ISH of E9.5 embryos using DIG-labeled LNA probes for miR-3099 (C and D) (n = 3) and miR-scrambled (E and F) (n = 2). Cryosection of the stained embryos shows expression of miR-3099 in the neuroepithelium of the telencephalon (D, inset in C). BA = branchial arches, DH = developing heart, FLB = forelimb bud, HLB = hindlimb bud, Mes = mesencephalon, NE = neuroepithelium, SO = somite, Tel = telencephalon. The mean ± SE for each tissue is presented in the bar graphs. Asterisks denote the statistical significance level at P < 0.01 (**) and P < 0.001 (***) based on the one-way ANOVA test (see Additional file 14 for analysis details).