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Figure 3 | BMC Genomics

Figure 3

From: Expansion and functional diversification of a leucyl aminopeptidase family that encodes the major protein constituents of Drosophila sperm

Figure 3

Annotation of S-LAPs on 2D gels of purified sperm. Gels were run using duplicate samples of solubilized sperm protein (100 ug/gel). Proteins were first separated on isoelectric focusing gels with pH ranges of (A) 4-7, (B) 6-11 and (C) 3-10 followed by second dimension SDS-PAGE using 12% Bis-Tris reducing gels. Spots were visualized using Coomassie Blue R250 and spots excised and submitted for MALDI-TOF and tandem MS/MS analyses. The α- and β-tubulins are indicated in (A) at the expected pI and molecular weight positions. Regions of the gel identified as S-LAPs are indicated by brackets with individual spots circled.

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BMC Genomics

ISSN: 1471-2164

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