Table 2 Summary of BAC library screening results with previously mapped microsatellites using PCR super-pools
From: A first generation integrated map of the rainbow trout genome
INRAa | USDAb | Combined | |
|---|---|---|---|
No. of markers tested | 265 | 137 | 396 |
Localized to a single FPC contig | 98 | 41 | 135 |
Localized to two FPC contigs | 7 | 5 | 11 |
Singletons or failed DNA fingerprinting | 21 | 15 | 35 |
Not validated by single clone PCR | 4 | 12 | 16 |
Not positive by PCR screening of superpools | 135 | 64 | 199 |
No. of chromosomes covered | 29 | 22 | 29 |