Table 4 Name of the genes, primers used and expected size of the RT-PCR products for amplification of full-length transcripts
S.No | Gene Name | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
1 | Acyl-CoA binding protein (ACBP) | CCCGGATACGTTCTATAAATC | CCCACACGCAAGAGAAGACACTA | 524 |
2 | Acyl carrier protein (ACP) | GAACTCTCGCTACTCTCTTTC | GCATAAATTAGGAAATTTTAGAGTGT | 641 |
3 | Biotin carboxyl carrier protein I of ACCase (BCCP I) | CGGAAATTACAAAGACAGACAC | TGTTTATAGCTTCACTAGTGTAC | 1220 |
4 | Biotin carboxyl carrier protein II of ACCase (BCCP II) | GAGGTTTATGGAGTTGTAACTG | TAATGATAAATAACAATGAAGAAGGC | 1316 |
5 | 3-keto acyl ACP Reductase (KAR) | ACAGACACATTCCTTTCTTCG | CACAGTATCTGTCACCTTTTC | 1417 |
6 | Acyl ACP thioesterase (FATA) | CTGACATTGGTCTTGAGCTCC | AGTAAACGTAAAACAATACAGTTGAT | 1448 |
7 | Delta12-fatty acid desaturase (Δ-12 FAD) | ACCGAGCAGAGGCCGGTATC | CTCTGAATTTGAATTTTTATAAACC | 1501 |
8 | Acyl-CoA thioesterase (ACT) | CCGGAGCCTGGACTACTGTT | ACCCCGGCTGCTAGAATCAA | 1535 |
10 | Diacyl Glycerol kinase (DGK) | CTATCTTCTTCAATACAAGTACG | GATTTTTGATACAACAAATTACCAGT | 1709 |
9 | 3-keto acyl CoA synthase (KCS) | CCGATCAATATTGAAGCAAAT | ACCAGCACAAACAATCCCAG | 1715 |
12 | ω-3-fatty acid Desaturase (ω-3-FAD) | GAAGACAATAAGCACAAATTG | CCCTCTCAAATCCAATCCAA | 1741 |
11 | Stearoyl-ACP desaturase (SAD) | CCCACTTTGATGCGTCTGCT | GACCTCCTGCTCGAGAATAGA | 1769 |
13 | Beta-keto acyl ACP synthase I (KASI) | GTAGCTCAATTTGCTGCGCCT | GCAGAAGAACACAATTTTGATAC | 1908 |
14 | Biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase (BC) | ACCGCATTCAATCTTTCTCAC | GACTTCATCAACTCACTTCTA | 1944 |
15 | Pyruvate Kinase (PK) | ACCGAGGTTTCGGTCTTCACC | GACCTGCCTGCCATGGCATTC | 1974 |
16 | PHD finger family protein | ATCGTGTTCAATTTAGGATTGAG | TCTGGAGTCTTGTTCTGGGTA | 4512 |
17 | Kinase interacting family protein | GTACCTTATGTTCTGAATGATGA | TGAAGTCCTTCAAGATGACAC | 5030 |