Transcriptomic analysis of milk somatic cells in mastitis resistant and susceptible sheep upon challenge with Staphylococcus epidermidis and Staphylococcus aureus

Table 3 RT-qPCR of the differentially expressed genes between resistant and susceptible lines

Microarray result	Genes	All data		Sa data		Se data
		Resistant	Susceptible	Resistant	Susceptible	Resistant	Susceptible
	CRYL1	26.50 ± 29.98**	4.65 ± 5.68	40.39 ± 37.92**	6.20 ± 6.66	12.61 ± 8.71*	3.10 ± 4.57
	TP53			4.53 ± 3.51**	1.16 ± 0.72
	RARα	1.34 ± 0.82**	0.76 ± 0.37	0.95 ± 0.58*	0.51 ± 0.31	1.72 ± 0.90*	1.02 ± 0.20
	SLC40A1			5.07 ± 8.07*	1.29 ± 1.10
Main List	GTPBP4			0.59 ± 0.47*	1.21 ± 0.92
	TMEM87B			0.73 ± 0.14*	1.19 ± 0.86
	PPAPDC1B	0.43 ± 0.39	0.81 ± 0.92	0.25 ± 0.13	0.26 ± 0.19	0.61 ± 0.50*	1.36 ± 1.05
	EIF4EBP1	1.54 ± 0.80	1.27 ± 0.75	1.93 ± 0.75	1.49 ± 1.01	1.14 ± 0.67	1.05 ± 0.33
	MAPRE1			0.87 ± 0.57	1.16 ± 0.76
	SAA2			0.33 ± 0.31**	1.86 ± 2.30
	ITGB6			0.42 ± 0.31**	1.49 ± 1.49
Sa list	S100A2			0.58 ± 0.39*	1.28 ± 0.94
	CCL5			0.55 ± 0.36*	1.35 ± 1.02
	TLR2			2.04 ± 2.17	1.09 ± 0.47
	CAPN3	3.63 ± 2.71**	1.52 ± 0.98	4.07 ± 3.23	1.66 ± 0.81	3.19 ± 2.28*	1.38 ± 1.18
Se list	PSMD4	0.82 ± 0.28**	1.06 ± 0.31	0.96 ± 0.27	1.08 ± 0.33	0.67 ± 0.22**	1.04 ± 0.32
	ST3GAL4	1.56 ± 1.31**	0.82 ± 0.50	0.96 ± 0.73	0.54 ± 0.37	2.16 ± 1.55**	1.10 ± 0.48

The results represent the mean ± standard deviation of the relative expression in qPCR of seventeen differentially expressed genes identified in the microarray analyses with all data (main list), S. aureus (Sa list) or S. epidermidis data (Se list). qPCR were performed with Sa or Se samples or with both Sa an Se samples (All data). A non parametric Wilcoxon test was performed with SAS to identify the differentially expressed genes. ** p-value < 0.05; * p-value < 0.10.

ISSN: 1471-2164