The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
- Kristin Baumann†1,
- Laura Dato†2,
- Alexandra B Graf†3, 4,
- Gianni Frascotti2,
- Martin Dragosits3, 6,
- Danilo Porro2,
- Diethard Mattanovich3, 5,
- Pau Ferrer1Email author and
- Paola Branduardi2
© Baumann et al; licensee BioMed Central Ltd. 2011
Received: 13 December 2010
Accepted: 9 May 2011
Published: 9 May 2011
Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations and adaptations which occur in their natural surroundings, but also in industrial processes. Notably, the majority of such transcriptome analyses were performed using non-engineered strains.
In this comparative study, the gene expression profiles of S. cerevisiae and P. pastoris, a Crabtree positive and Crabtree negative yeast, respectively, were analyzed for three different oxygenation conditions (normoxic, oxygen-limited and hypoxic) under recombinant protein producing conditions in chemostat cultivations.
The major differences in the transcriptomes of S. cerevisiae and P. pastoris were observed between hypoxic and normoxic conditions, where the availability of oxygen strongly affected ergosterol biosynthesis, central carbon metabolism and stress responses, particularly the unfolded protein response. Steady state conditions under low oxygen set-points seemed to perturb the transcriptome of S. cerevisiae to a much lesser extent than the one of P. pastoris, reflecting the major tolerance of the baker's yeast towards oxygen limitation, and a higher fermentative capacity. Further important differences were related to Fab production, which was not significantly affected by oxygen availability in S. cerevisiae, while a clear productivity increase had been previously reported for hypoxically grown P. pastoris.
The effect of three different levels of oxygen availability on the physiology of P. pastoris and S. cerevisiae revealed a very distinct remodelling of the transcriptional program, leading to novel insights into the different adaptive responses of Crabtree negative and positive yeasts to oxygen availability. Moreover, the application of such comparative genomic studies to recombinant hosts grown in different environments might lead to the identification of key factors for efficient protein production.
Yeasts are very well established host systems for the production of a broad number of recombinant proteins, and they are easy to manipulate and cultivate. During the last decades, many efforts have been made to tap their potential for improving foreign protein production for both commercial and academic purposes (for recent reviews, see [1, 2]). Although a great number of such manipulations have been successful, these efforts were essentially based on the existing knowledge and empirical methods [3, 4]. The initiation of the genomics era and the deciphering of the Saccharomyces cerevisiae genome in 1996 have enabled a significant progress in the identification of genes that were involved or activated in response to a wide range of different stresses [5, 6], but only a few studies took advantage of the new systems biology tools to uncover novel helper genes to enhance heterologous protein secretion [3, 7–9]. Until recently, even less effort has been made to combine such studies to investigate the impact of environmental stress responses (ESR) on recombinant protein production. Cells undergo continuous environmental fluctuations during industrial processes, triggering physiological responses and adaptations which are highly interrelated with correct protein folding and secretion. It is thus obvious that such studies - combining environmental stress responses under protein production conditions - would provide an important platform to identify targets for a rational engineering of not only yeasts but also other cellular protein factories.
The recently published sequence of the Pichia pastoris genome [10, 11] allowed the development of P. pastoris specific microarrays  and the onset of genome-wide studies in this yeast expression system, which has been gaining high industrial relevance in the recent years. This advance was of special importance because the lack of host specific microarrays not only hindered research, but also implicated a certain dependency of data interpretation on similarities to S. cerevisiae. Recently, Dragosits and co-workers investigated the effects of temperature  and osmolarity  on the proteome/fluoxome and proteome/transcriptome, respectively, of a recombinant P. pastoris strain. Notably, the application of different levels of osmolarity to growing P. pastoris cells demonstrated differences in the physiological response as compared to published data for S. cerevisiae. Similarly, important differences between these yeasts have also been observed for the unfolded protein response (UPR) upon dithiothreitol (DTT) treatment and in a strain over-expressing the UPR transcription factor HAC1. These differences between data on P. pastoris and the established literature of S. cerevisiae point to less uniform regulatory systems in yeasts than believed until now.
The basis of this genome-wide comparative study were the previously analyzed transcriptional responses of recombinant S. cerevisiae (unpublished results) and P. pastoris to oxygen availability. The engineered yeasts and their corresponding control (empty vector) strains were grown in normoxic, oxygen-limited and hypoxic conditions in glucose-limited chemostats. Both recombinant strains secreted a human antibody Fab fragment [16, 17] under control of the constitutive glycolytic promoters pGAP (glyceraldehyde-3 phosphate dehydrogenase, for P. pastoris) and pTPI (triosephosphate isomerase, for S. cerevisiae).
Together with the relevance for industrial processes, where oxygen transfer is often described as important issue in high cell density fermentations, oxygen is of special interest in the comparison of two yeasts, both facultative anaerobe but with different capacities to ferment glucose (Crabtree effect). While S. cerevisiae is a highly fermentative Crabtree positive yeast, able to produce ethanol aerobically in the presence of high concentrations of glucose, P. pastoris is Crabtree negative and more sensitive to the availability of oxygen than to variations in substrate concentration.
Also, it is well known that environmental conditions have an effect on recombinant protein secretion, and that the profile and severity of such effect is host dependent. We are still far away from an ideal scenario of a universal expression system, like proposed by Sørensen . However, a genome-wide approach aiming at the understanding of the global mechanisms connecting protein production to environmental conditions, as proposed in this study, could help to find strain or species dependent target genes or target mechanisms for cell engineering.
Results and Discussion
Physiological changes in yeasts under different levels of oxygen availability
YDM [g l-1]
Fab yield [mgFab3H6 gYDM-1]
ethanol [g l-1]
arabitol a or glycerol b [g l-1]
9.01 ± 0.24
8.69 ± 0.19
5.88 ± 0.23
0.027 ± 0.005
0.029 ± 0.005
0.023 ± 0.004
0.06 ± 0.017
3.64 ± 0.36
9.14 ± 0.52
8.59 ± 0.28
5.13 ± 0.31
0.05 ± 0.007
3.46 ± 0.33
23.98 ± 0.67
22.54 ± 1.06
12.58 ± 1.12
0.22 ± 0.01
0.38 ± 0.01
0.54 ± 0.02
0.89 ± 0.09
6.85 ± 0.19
0.90 ± 0.07
2.88 ± 0.18
23.61 ± 0.77
20.14 ± 0.59
11.68 ± 0.28
1.14 ± 0.12
5.75 ± 0.08
1.21 ± 0.21
2.19 ± 0.26
General overview of the transcriptional analysis
Principal component analysis
Gene ontology group representation
In case of the comparison hypoxic vs. fully aerobic, differences between the yeast species could be observed for the GO terms cell cycle, chromosome segregation, response to oxidative stress and translation. These four groups were enriched with downregulated genes (green) in S. cerevisiae and upregulated genes (red) in P. pastoris. For the functional categories cellular homeostasis, amino acid metabolic process, protein catabolic process, conjugation, mRNA metabolic process, peroxisome organization and biogenesis, response to stress and vitamin metabolic process, a significant number of genes were either downregulated in P. pastoris, and/or genes with a positive fold change were overrepresented in S. cerevisiae. Patterns of similarly regulated GO groups in the two yeast species could be observed for cofactor metabolic process, protein transport and RNA metabolic process, all of them comprising gene sets with both-side directed regulation (orange). A strain-dependent behavior (control vs. expressing) was recognized for the GO categories chromosome organization and biogenesis, DNA metabolic process, vesicle mediated transport and tRNA metabolic process. While the first three groups were upregulated in the expressing strains and bidirectional in the reference strains, the tRNA metabolic process was significantly downregulated in the control strains. Additionally, for the categories cellular lipid metabolic process, generation of precursor metabolites and energy, mitochondrion organization and biogenesis and ribosome biogenesis the behaviour of both S. cerevisiae strains (recombinant and control) and of the P. pastoris Fab-expressing strain was similar, but different in the P. pastoris control strain.
Omics Expression Viewer
An interesting observation was the opposite regulation of members of the ergosterol biosynthesis pathway. Ergosterol is an essential component of membrane lipids and regulates the fluidity and permeability of the plasma membrane, where it is most abundant. It has a broad function in cellular processes like plasma membrane fusion, pheromone signaling or protein sorting [24–26]. Furthermore, ergosterol biosynthesis is an oxygen-requiring process. In P. pastoris the majority of genes whose products catalyze oxygen-dependent reactions of this pathway (ERG1, ERG11, ERG25 and ERG3) were strongly upregulated in hypoxia, while ERG27, ERG6 and ERG4 were downregulated. In contrast, all the mentioned genes resulted to be downregulated in S. cerevisiae. We recently hypothesized that the upregulation of the genes catalyzing oxygen-consuming reactions in the ergosterol pathway of P. pastoris may be a reaction to intermediate or end product deficit provoked by low oxygen availability , since the ergosterol content was shown to be reduced under hypoxic conditions in a parallel study . A similar trend was described for S. cerevisiae[28, 29]. It has been assumed that the anaerobic/hypoxic induction of genes catalyzing oxygen-dependent reactions in some cases could be an adjustment of cells to subsequent oxygenation of the cultivations. In the case of S. cerevisiae, the ergosterol regulatory process may depend on the sterol requirement for growth and on the presence of other lipids, as postulated by Rosenfeld and Beauvoit . In this context we assume that the sterol content in S. cerevisiae under hypoxic conditions was still sufficient to maintain growth. Interestingly, Rintala and co-workers  observed a similar downregulation of ERG11, ERG25 and ERG6 with 2.8% oxygen (in the inlet air) when compared to full aeration, but unchanged levels of ERG1, ERG3 and ERG27. Unlike in this work, however, their growth medium was supplemented throughout the study with ergosterol and unsaturated fatty acids, which are typically supplied only to anaerobic S. cerevisiae cultivation media in order to sustain growth [32, 33]. Since endogenous or exogenous sterols are regulators of ERG gene expression, the provision of exogenous ergosterol in such experiments might have partially masked the "authentic" hypoxic response of S. cerevisiae and could explain the observed dissimilarities. In addition, transcriptional regulation could be directly influenced by oxygen concentration. Bunn and Poyton  demonstrated that different genes responding to the presence of oxygen have different thresholds for activation/deactivation of their transcription. In this view, it could be possible to imagine that different thresholds are sensed in the two yeasts, consistently with their different fermentation capacities. Therefore, a condition of oxygen limitation might be sensed by P. pastoris as more extreme, eliciting a proper response.
In relation to protein secretion, there are some indications that lipid metabolism, especially with regard to ergosterol biosynthesis and membrane remodeling, may affect protein secretion. This pathway was discussed in the recent work of our group as possible target for strain engineering, since it encountered a very strong reorganization during hypoxic conditions in which the secretion of the antibody Fab fragment was significantly improved . Recent findings in our lab showed that disequilibrium of the membrane properties by applying non-ionic detergents or gene specific antifungal drugs stimulated recombinant protein secretion in P. pastoris shake flask cultures (Baumann et al., submitted). These findings strongly support our hypothesis about a link between changes in membrane fluidity and recombinant protein secretion in P. pastoris. Nonetheless, although an increased membrane fluidity was demonstrated for erg mutants of S. cerevisiae with defective ergosterol biosynthesis , such kinds of mutants were reported to be affected in the sorting of proteins to the plasma membrane  and in protein internalization via endocytosis  but not in protein secretion [36, 37]. Therefore, it might be that differences in the membrane composition due to ergosterol limitation and/or compensation mechanisms do not have the same influence on protein secretion mechanisms in the two yeasts, thus contributing to the different secretion capacity observed in hypoxic conditions.
Transcription of the pentose phosphate pathway (PPP) genes was significantly reduced in both yeasts, but to a greater extent in S. cerevisiae. While in P. pastoris only genes of the non-oxidative pathway were regulated (RKI1, TKL1 and TAL1), in S. cerevisiae this list was supplemented by genes from the oxidative PPP branch including SOL3, SOL4, GND1, GND2. It was previously described that the distribution of carbon flux between glycolysis and PPP seems to be associated with the ability of yeasts to perform aerobic fermentation (Crabtree effect) [38, 39]. S. cerevisiae as a Crabtree positive yeast was shown to have rather low metabolic fluxes through the pentose phosphate pathway  which is predominantly used for NADPH production and less directly for intermediates production. The downregulation of the pathway under hypoxic conditions may prevent an imbalance in the cellular redox system, since reducing equivalents like NADH or NADPH are accumulated in anaerobic cultivations. While S. cerevisiae typically produces glycerol in order to re-oxidize excess NADH , P. pastoris was recently shown to circumvent redox imbalances by the production of arabitol . This observation could give reason for the different regulation, since D-ribulose and D-xylulose from the upper (oxidative) PPP are the main precursors for the formation of arabitol in many fungi [42, 43].
Clusters of interest
Cluster 2 comprised 4 mating related genes (FIG1, KAR4, SST2 and STE2), which were strongly upregulated in all pair wise comparisons in S. cerevisiae, and downregulated in P. pastoris. This result may reflect the different life cycle of the yeasts, which alternates between haplophase and diplophase in S. cerevisiae, while P. pastoris is most stable in its vegetative haploid state. In S. cerevisiae, mating occurs between two haploid cells of opposite mating type MATa or MATα (reviewed in ) after pheromone signalling, resulting in a diploid nucleus. In P. pastoris mating occurs only in conditions of severe nitrogen limitation .
The expression pattern in Cluster 3 (13 members) showed a species dependent regulation and had its strongest impact on the transcriptome of P. pastoris comparing hypoxic vs. normoxic and oxygen-limited vs. normoxic conditions. Among the most regulated genes we found 4 peroxisomal genes (PCD1, PEX11, PEX13 and the glyoxylate cycle involved gene MDH3) and 2 genes that are related to glycerol metabolism and transport (GUT1, YFL054C). S. cerevisiae may have a higher tolerance for oxygen deficiency by maintaining peroxisomal activities and restoring biosynthetic intermediates via anaplerotic reactions (e.g. glyoxylate cycle) . On the other hand, carbon source may impact regulation of peroxisomal genes in the methylotrophic yeast P. pastoris. Since peroxisomes harbour the enzymes for methanol assimilation, these organelles drastically increase in size during growth on methanol, but degrade rapidly upon a shift to glucose . This would, however, only explain a general low transcriptional level of peroxisome-related genes in P. pastoris grown on glucose, but not a downregulation under hypoxic and oxygen-limited conditions. We therefore assume an implication of other oxygen-related processes, e.g. the breakdown of fatty-acids, which is drastically reduced when oxygen is scarce (see cluster 4). GUT1, whose expression is induced on non-fermentable carbon sources such as glycerol or ethanol, and YFL054C, which encodes a putative channel-like protein that mediates passive diffusion of glycerol in the presence of ethanol, were slightly induced in S. cerevisiae at low oxygen availability. Since glycerol is generated in anaerobic cultures for the purpose of re-establishing the cell's redox balance, the upregulation of these genes is reasonable.
Further genes found in this cluster were two iron-related genes (the transcription factor AFT1 and the iron transporter SIT1), the a-mating factor receptor STE3, and a cell wall mannoprotein which is required for growth under anaerobic conditions (TIR3).
Cluster 4 contained 6 fatty acid β-oxidation pathway related genes (ECI1, FAA2, FOX2, POX1, SPS19 and PXA1). The transcript pattern, with a similar trend in both yeast species, indicated a strong downregulation in the set-points with low oxygen availability, but a slight upregulation in the comparison HvL, that is, showing their lowest gene expression levels under oxygen-limited rather than hypoxic conditions. Interestingly, the transcript levels were generally lower in the Fab-producing strains.
Cluster 5 included 175 genes with the majority of them downregulated in P. pastoris under hypoxic and oxygen-limited conditions. While the genes for S. cerevisiae were marginally induced when comparing hypoxic with aerobic conditions, the profile was rather unchanged for the other pairwise comparisons. This cluster was enriched with TCA cycle genes (KGD2, SDH4, LSC1, LSC2, CIT1 and FUM1) and genes involved in amino acid biosynthetic process (LYS21, GLT1, ILV2, SER2, HIS6, and ARO7). The downregulation of the TCA cycle in hypoxic P. pastoris chemostat cultivations on the transcriptome, proteome and metabolome levels was recently reported by our group . While in S. cerevisiae, a similar repression of TCA cycle transcripts was observed for anaerobic glucose-limited chemostats [23, 48, 49], intermediate oxygen conditions with 2.8 % oxygen in the inlet gas induced a moderate upregulation of the transcripts for the main TCA cycle enzymes , which is in accordance with the results in this study. In this context, the similar regulation of the amino acid biosynthetic genes may be partly interrelated, since the TCA cycle provides biosynthetic precursors for amino acids including lysine (LYS21), glutamine (GLT1) and isoleucine (ILV2) .
A set of genes was associated with response to oxidative (GRX3, DOT5, SRX1, CCP1) and DNA damage stress (MSH3, HAM1, YNK1). Aerobically grown cells are always exposed to some degree of oxidative stress, and activate defence mechanisms in order to repair (or to prevent further) damage. It thus seems plausible that P. pastoris silences the oxidative stress defences in hypoxic and oxygen-limited conditions as compared to normoxia. Interestingly, the transcript levels of such stress related genes in hypoxically grown S. cerevisiae were slightly induced. It is very unlikely that a surplus of reducing equivalents in low oxygen environments allows for the generation of radicals. Krantz and co-workers  observed a similar trend in S. cerevisiae chemostats under anaerobic osmo-stress conditions, and assumed a co-regulation with general stress response genes (like CCT1), however, without any evident requirement.
Three glycogen biosynthetic genes (GLG1, GSY2, and GAC1) were also present in cluster 5. Glycogen, a highly abundant reserve compound in yeasts and composed of glucose, is generally considered to provide both carbon and energy during starvation periods (extensively reviewed in ). P. pastoris' glycogen content was shown previously to be higher in aerobic conditions than in conditions of low oxygen provision , which coincided with the observed downregulation of the related genes under oxygen limitation. It seems plausible that P. pastoris cells do not synthesize glycogen in glucose-limited hypoxic cultures, but rather mobilize stored glycogen for energy purposes and the conservation of a high glycolytic flux. The unchanged/slightly induced transcripts of glycogen-synthetic genes in S. cerevisiae during hypoxia, in contrast, remain unclear, but could be related to the involvement of glycogen accumulation during spore formation in diploid cells . Evidence for increased spore formation in hypoxically grown S. cerevisiae is given by the induced genes associated with ascospore formation in cluster 9.
Another functional category that is enriched in cluster 5 consists of five transcription factors controlling various cellular functions, like mating (HMRA1, STE12), ethanol, glycerol and fatty acid utilization and peroxisomal gene transcription (ADR1), nucleotide excision repair (TFB3) and phospholipid biosynthesis (OPI1). A number of known targets of these transcription factors were also present in the downregulated clusters.
Cluster 7 contained genes with a low level of expression in S. cerevisiae and with induced transcripts in P. pastoris in hypoxic and oxygen-limited conditions, as compared to normoxic conditions. This cluster was considerably enriched with genes of the ergosterol pathway (ERG3, ERG4, ERG5, ERG6, ERG24, ERG25, ERG26 and ERG27). As discussed before (see Omics expression viewer Section), there are some differences on ergosterol regulation in S. cerevisiae under anaerobic or oxygen-limited conditions . Additionally, the usual supplement of ergosterol to anaerobically grown S. cerevisiae hampers a direct comparison with our data, since we did not add ergosterol in any oxygen condition. The results of this comparative analysis, however, clearly indicate remarkable differences in the regulation of sterol pathways in the two yeasts in a reduced oxygen environment.
Two trehalose metabolic genes (TPS2 and ATH1) were also found in this cluster. Tps2p catalyzes the synthesis of the reserve compound trehalose, which is also known as stress protecting agent (reviewed in ) that has an extraordinary capacity of stabilizing proteins and preventing aggregation of denatured proteins . Chen et al.  observed that trehalose accumulation in Drosophila protected the cells from hypoxic injury. However, in a recent study on the macromolecular and elemental composition of P. pastoris grown under the exactly same experimental conditions , no trehalose accumulation could be detected when varying the oxygenation degree or the strain genetic background. Since ATH1 is involved in the catabolism of trehalose, it appears that the trehalose pool might have a higher turnover in hypoxic and oxygen-limited P. pastoris cultivations. In agreement with the transcriptional profiles, instead, the trehalose content of S. cerevisiae decreased when reducing oxygen supply (data not shown), as similarly reported elsewhere .
The regulation of genes encoding enzymes capable of xylose reduction (GCY1 and YJR096W) appears to be characteristic for P. pastoris. Xylose reduction is most probably involved in the formation of arabitol in P. pastoris hypoxic fermentations. Another gene of special interest in this cluster was related to protein secretion (NCE102) since it is involved in non-classical protein export . Similarly, we recently identified NCE103, encoding a substrate for the non-classical protein export pathway, to be hypoxically induced in P. pastoris, with a significantly stronger upregulation in the producing strain .
Cluster 9 (26 members) is primarily characterized by strongly induced mRNA levels in S. cerevisiae and unchanged or slightly downregulated genes in P. pastoris hypoxic and oxygen-limited conditions, as compared to fully aerobic conditions. Some exceptions showed a uniform upregulated pattern for all pairwise comparisons (FSH1, GSA2, PHO84 and WSC4) or a strong upregulation in the P. pastoris control strain (YCT1). OPT2, an oligopeptide transporter with a role in vacuole assembly, and CDA2, ascospore wall formation, showed a stronger induction in the S. cerevisiae producing strain. The cluster was predominantly composed of ascospore formation genes (CDA2, GAS2, SPO75, SPR1, SPR3 and SPS2) and transmembrane transporter genes (DAL4, DAL5, YCT1, PHO84, FET4 and OPT2). As already discussed before, spore formation mainly derives from diploid cells, which is a very uncommon state in the life cycle of P. pastoris. Spore formation, however, is also known to be a strictly aerobic process because it only takes place in the presence of a poor carbon source and in cells starved of nitrogen (, and reviewed in ). Therefore, the upregulation of some spore formation associated genes might be related to other adaptive processes towards low oxygen availability or rather alert the cells to prepare them for more environmental extremes.
DAL4 and DAL5 are transporters of allantoin - an intermediate of adenine and guanine degradation that can serve as sole nitrogen source. The induction of these genes in S. cerevisiae could be a hint to a higher need of nitrogen under hypoxic conditions as compared to P. pastoris. Also, the upregulation of some transmembrane transporters may indicate some extra demand in S. cerevisiae for important building blocks like metals, amino acids or oligopeptides.
Cluster 10 was mainly composed of glycolytic genes (CDC19, ENO1, EXG1, GLK1, GPM1, PFK1, PFK2, PGK1, and TDH3) and stress-related genes (UGA2, YDL124W, HSP12, TSA1 and NCE103). Furthermore, it included two important protein folding-associated genes (ERO1 and HAC1) and three ergosterol genes (ERG1, ERG11 and ERG28). The pattern in this cluster was predominantly characterized by a strong induction in HvN and LvN in P. pastoris strains. The transcriptional induction of glycolysis and ergosterol synthesis in P. pastoris under low oxygen conditions has already been discussed in the "Omics expression viewer" section. Among the stress related genes, NCE103 is known to have a protective role in the presence of H2O2, but also a participation in a non conventional protein export, as observed for NCE102, has been proposed . UGA2, a succinate semialdehyde dehydrogenase with a function in gamma-aminobutyric acid (GABA) degradation, was also reported to be induced by H2O2 thus increasing oxidative stress tolerance . However, it is very unlikely that hypoxia triggered such antioxidant responses, since no respiratory activity could be detected in the case of P. pastoris. We rather propose some other function of their gene products, like the involvement of Nce103p in protein secretion as previously mentioned, or the role of Uga2p in degrading the non-protein amino acid GABA for nitrogen utilization. A further "moonlighting" protein with multiple functions is the gene product of TSA1, which plays a role as antioxidant under normal conditions , but self-associates to form a chaperone complex as a consequence of environmental perturbations. In its chaperone state it targets unfolded proteins in order to prevent their aggregation . HSP12, another chaperone-encoding gene that was induced by hypoxia in P. pastoris, confers increased stability to membranes in the presence of ethanol.
Many chaperones are helper factors throughout the protein folding process and assure the release of only correctly folded polypeptides from the endoplasmic reticulum (ER). Environmental stresses or similar events can easily provoke an overload of the ER folding machine, resulting in the aggregation of unprocessed proteins and consequently in the onset of the unfolded protein response (UPR) (reviewed in ). Transcripts of the transcription factor activating this cellular mechanism, Hac1p, were also significantly induced in P. pastoris as compared to S. cerevisiae. Notably, unlike in P. pastoris, S. cerevisiae HAC1 is not regulated at the transcriptional level upon UPR, but only by splicing of the transcript (HAC1s). This means that comparing UPR induction in both yeasts can be done only on a basis of UPR target genes like KAR2, PDI1 and ERO1. Since none of these genes was upregulated in S. cerevisiae, we could exclude any UPR activity in this yeast, which would explain also why other chaperones like TSA1 and HSP12 were unchanged as well. In good consistency to the overall results of this study, HAC1 regulation could also reasonably be linked to changes in the lipid balance, since UPR was also suggested to be activated upon lipid deprivation in order to coordinate membrane synthesis, with Hac1 as important regulator . Since we also observed a drastic difference between the yeasts in the regulation of ergosterol biosynthesis, alterations in membrane fluidity could have provoked the upregulation of P. pastoris HAC1.
In good agreement with these results, also ERO1, required for oxidative protein folding in the endoplasmic reticulum, was activated upon hypoxia in P. pastoris. Together with PDI1, another key player during UPR, it catalyzes the transfer of oxidizing equivalents to folding proteins . It is likely that the differences in the regulation of the protein folding machinery are reflected in the different protein product yields in P. pastoris and S. cerevisiae, since a number of studies have reported a stimulatory effect of the overexpression of UPR related genes (e.g. PDI1, HAC1s and ERO1) on recombinant protein secretion .
It is apparent from this work that there are a number of pathways that are not overlapping in their response to oxygen availability in these two yeasts, thus reflecting important differences in the regulation of their metabolisms.
The effect of three different conditions of oxygen provision on the transcriptome of P. pastoris and S. cerevisiae revealed a very distinct remodelling of the genomic expression program, particularly in the case of hypoxia. On one side, such observed differences point to a different importance of transcriptional regulation mechanisms in respect to metabolic remodelling following adaptation to reduced oxygen availability, which probably reflect a divergence in the evolution of control mechanisms preferred by the two yeasts. This might be related to different strategies for cell survival in the competition with other microorganisms: For example, a preference towards post-transcriptional or post-translational regulation mechanisms, as inferred for S. cerevisiae, gives the possibility of a faster adaptation to changing conditions and, therefore, more versatility. On the other side, clear differences between the two yeasts were detected at the transcriptome level for several cellular processes related with protein secretion, namely the ergosterol biosynthesis pathway, the central carbon metabolism and the stress and unfolded protein response. Such physiological differences underlying the distinct impact of hypoxia on protein secretion in the two yeasts give a strong indication on those pathways whose regulation/manipulation might lead to improved phenotypes with respect to heterologous protein production under well defined environmental conditions.
Overall, the results of this study indicate that combination of systems biology approaches and biological systems diversity is a useful tool to gain novel insights into the physiology of biotechnologically important microorganisms, from which new strategies for rational optimization of strains and cultivation conditions could be derived.
Strains and chemostat cultivations
The construction of the P. pastoris strains used in this study as well as a detailed description of the chemostat cultivations and sampling are reported elsewhere . In brief, a recombinant P. pastoris X-33 (wild type phenotype) derived strain and its corresponding empty-vector control strain were cultivated in a glucose-limited chemostat at a dilution rate of 0.1 h-1. The recombinant strain secreted the light and heavy chain of a human monoclonal antibody Fab (3H6) fragment under the constitutive GAP promoter. Cultivations were performed at 3 different oxygen concentrations in the inlet gas stream, yielding normoxic (21 % O2), oxygen-limited (11 % O2) and hypoxic (8 % O2) conditions. Triplicate samples for the DNA microarray experiments were taken at steady state conditions for each oxygen set-point.
The coding sequences for the 3H6 Fab heavy and light chain were both integrated on a pYX integrative expression vector under control of the constitutive S. cerevisiae TPI1 promoter. The S. cerevisiae α-factor leader sequence was used to target the protein into the supernatant, and a polyA sequence served as terminator. The expression vectors were integrated into the genome of S. cerevisiae CEN.PK strain with HIS3 and URA3 as selection markers. The control strain was transformed with the empty pYX vectors.
Chemostat cultivations were performed in a bench-top bioreactor (Biostat B, Braun Biotech International GmbH) at a working volume of 1.25 L. After a batch period of approximately 24 hours, chemostat cultivation was initiated at a dilution rate of D = 0.1 h-1. The parameters were set to 1000 rpm and 26°C, and the pH was controlled at 5.0. The total gas flow was kept constant for all experiments at 1 vvm (volume gas per volume medium and minute). While normoxic cultivations were run with pure air in the inlet gas, the oxygen concentration was reduced to 5 % and 2 % for the oxygen-limited and hypoxic conditions, respectively, by partially replacing the air with nitrogen. Samples were taken from three independent experiments after reaching steady state conditions, i.e. after at least 5 residence times τ.
The P. pastoris specific DNA microarrays used in this study were produced on the Agilent platform (Agilent Technologies) and established by Graf et al. . The transcriptome analysis for S. cerevisiae was carried out with Agilent Yeast Gene Expression Microarrays, 4 × 44K. For both yeast species, RNA extraction, cDNA synthesis and labeling, as well as the microarray hybridizations and data analysis were performed as reported in previous studies [12, 14]. All samples were labeled in a dye-swap manner and hybridized against a reference cDNA, which was generated from a pool of cells (either P. pastoris or S. cerevisiae) grown under different culture conditions. Microarray data are available in the ArrayExpress database http://www.ebi.ac.uk/arrayexpress under accession number E-MEXP-2742 for P. pastoris and E-MEXP-3134 for S. cerevisiae.
The common gene list between P. pastoris and S. cerevisiae contains orthologs of the two species. Orthology was determined using a reciprocal best hit strategy based on protein BLASTs of P. pastoris proteins against S. cerevisiae proteins and vice versa. The protein BLAST was performed with an E-value cut-off < 10-5 and the BLAST reports were filtered for hits that had at least one high scoring segment > 50 amino acids with a similarity > 40 %. Of the resulting list, 388 gene IDs had to be excluded since they were either missing on the S. cerevisiae or the P. pastoris microarray. Additional file 1 contains the final list of orthologs (2891 gene IDs) with an indication of the number of S. cerevisiae proteins for which the respective P. pastoris protein was the best hit. A complete account of the annotation of P. pastoris can be found in De Schutter et al.  and Mattanovich et al. .
Principal Component Analysis (PCA) and Fisher's exact test for identification of significantly regulated gene groups based on gene ontology classes (GO groups) were carried our using the R platform http://www.r-project.org. The selection of GO groups was based on the GOSlim terms with some very large classes being resolved in more detail. The absolute (normalized) expression values of each yeast species were applied in both analyses.
Fold change expression data were subjected to the 'Expression Omics Viewer' tool from the SGD Saccharomyces genome database http://www.yeastgenome.org for visualization of the metabolic gene regulation when comparing hypoxic with normoxic conditions. A three color display with a specified threshold was applied, with red for data values that exceed the threshold of 0.59 (log2 fold change, equivalent to a fold change of 1.5), yellow for data values less than the inverse of that threshold, and blue for values in between.
Data clustering and visualization were performed with the open source tool Expression Profiler from EBI http://www.ebi.ac.uk/expressionprofiler. A cluster comparison was run between hierarchical clustering (correlation-based distance, average linkage) and k-means clustering (Euclidean distance, k = 10). Main clusters were manually analyzed for the most relevant discriminatory GO groups and genes for each organism, strain or oxygen comparison.
This work has been supported by the European Science Foundation (ESF, program EuroSCOPE), the Spanish Program on Chemical Process Technologies (project CTQ2007-60347/PPQ) and the Complementary Actions Plan (project BIO2005-23733-E) supporting the ESF project, the Catalan Government (contract grant 2009-SGR-281, Xarxa de Referència en Biotecnologia and a doctoral fellowship for K.B.), the Austrian Science Fund (FWF), project I37-B03 and the Austrian Research Promotion Agency (Program FHplus), and project EU FP7 SYSINBIO project (212766) to P.B.
- Idiris A, Tohda H, Kumagai H, Takegawa K: Engineering of protein secretion in yeast: strategies and impact on protein production. Appl Microbiol Biotechnol. 2010, 86 (2): 403-417. 10.1007/s00253-010-2447-0.PubMedView ArticleGoogle Scholar
- Graumann K, Premstaller A: Manufacturing of recombinant therapeutic proteins in microbial systems. Biotechnol J. 2006, 1 (2): 164-186. 10.1002/biot.200500051.PubMedView ArticleGoogle Scholar
- Graf A, Dragosits M, Gasser B, Mattanovich D: Yeast systems biotechnology for the production of heterologous proteins. FEMS Yeast Res. 2009, 9 (3): 335-348. 10.1111/j.1567-1364.2009.00507.x.PubMedView ArticleGoogle Scholar
- Gasser B, Saloheimo M, Rinas U, Dragosits M, Rodríguez-Carmona E, Baumann K, Giuliani M, Parrilli E, Branduardi P, Lang C, et al: Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact. 2008, 7: 11-10.1186/1475-2859-7-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Gasch A, Spellman P, Kao C, Carmel-Harel O, Eisen M, Storz G, Botstein D, Brown P: Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell. 2000, 11 (12): 4241-4257.PubMed CentralPubMedView ArticleGoogle Scholar
- Causton H, Ren B, Koh S, Harbison C, Kanin E, Jennings E, Lee T, True H, Lander E, Young R: Remodeling of yeast genome expression in response to environmental changes. Mol Biol Cell. 2001, 12 (2): 323-337.PubMed CentralPubMedView ArticleGoogle Scholar
- Wentz A, Shusta E: A novel high-throughput screen reveals yeast genes that increase secretion of heterologous proteins. Appl Environ Microbiol. 2007, 73 (4): 1189-1198. 10.1128/AEM.02427-06.PubMed CentralPubMedView ArticleGoogle Scholar
- Sevastsyanovich Y, Alfasi S, Cole J: Sense and nonsense from a systems biology approach to microbial recombinant protein production. Biotechnol Appl Biochem. 2010, 55 (1): 9-28. 10.1042/BA20090174.PubMedView ArticleGoogle Scholar
- Gasser B, Sauer M, Maurer M, Stadlmayr G, Mattanovich D: Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microbiol. 2007, 73 (20): 6499-6507. 10.1128/AEM.01196-07.PubMed CentralPubMedView ArticleGoogle Scholar
- De Schutter K, Lin Y, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, Rouzé P, Van de Peer Y, Callewaert N: Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol. 2009, 27 (6): 561-566. 10.1038/nbt.1544.PubMedView ArticleGoogle Scholar
- Mattanovich D, Graf A, Stadlmann J, Dragosits M, Redl A, Maurer M, Kleinheinz M, Sauer M, Altmann F, Gasser B: Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb Cell Fact. 2009, 8: 29-10.1186/1475-2859-8-29.PubMed CentralPubMedView ArticleGoogle Scholar
- Graf A, Gasser B, Dragosits M, Sauer M, Leparc G, Tüchler T, Kreil D, Mattanovich D: Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics. 2008, 9: 390-10.1186/1471-2164-9-390.PubMed CentralPubMedView ArticleGoogle Scholar
- Dragosits M, Stadlmann J, Albiol J, Baumann K, Maurer M, Gasser B, Sauer M, Altmann F, Ferrer P, Mattanovich D: The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res. 2009, 8 (3): 1380-1392. 10.1021/pr8007623.PubMedView ArticleGoogle Scholar
- Dragosits M, Stadlmann J, Graf A, Gasser B, Maurer M, Sauer M, Kreil D, Altmann F, Mattanovich D: The response to unfolded protein is involved in osmotolerance of Pichia pastoris. BMC Genomics. 2010, 11 (1): 207-10.1186/1471-2164-11-207.PubMed CentralPubMedView ArticleGoogle Scholar
- Baumann K, Carnicer M, Dragosits M, Graf A, Stadlmann J, Jouhten P, Maaheimo H, Gasser B, Albiol J, Mattanovich D, et al: A multi-level study of recombinant Pichia pastoris in different oxygen conditions. BMC Syst Biol. 2010, 4 (1): 141-10.1186/1752-0509-4-141.PubMed CentralPubMedView ArticleGoogle Scholar
- Gach J, Maurer M, Hahn R, Gasser B, Mattanovich D, Katinger H, Kunert R: High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris. J Biotechnol. 2007, 128 (4): 735-746. 10.1016/j.jbiotec.2006.12.020.PubMedView ArticleGoogle Scholar
- Gach J, Quendler H, Strobach S, Katinger H, Kunert R: Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5. Mol Immunol. 2008, 45 (4): 1027-1034. 10.1016/j.molimm.2007.07.030.PubMedView ArticleGoogle Scholar
- Sørensen H: Towards universal systems for recombinant gene expression. Microb Cell Fact. 2010, 9: 27-10.1186/1475-2859-9-27.PubMed CentralPubMedView ArticleGoogle Scholar
- Baumann K, Maurer M, Dragosits M, Cos O, Ferrer P, Mattanovich D: Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol Bioeng. 2008, 100 (1): 177-183. 10.1002/bit.21763.PubMedView ArticleGoogle Scholar
- Raychaudhuri S, Stuart J, Altman R: Principal components analysis to summarize microarray experiments: application to sporulation time series. Pac Symp Biocomput. 2000, 455-466.Google Scholar
- Daran-Lapujade P, Jansen M, Daran J, van Gulik W, de Winde J, Pronk J: Role of transcriptional regulation in controlling fluxes in central carbon metabolism of Saccharomyces cerevisiae. A chemostat culture study. J Biol Chem. 2004, 279 (10): 9125-9138. 10.1074/jbc.M309578200.PubMedView ArticleGoogle Scholar
- de Groot M, Daran-Lapujade P, van Breukelen B, Knijnenburg T, de Hulster E, Reinders M, Pronk J, Heck A, Slijper M: Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post-transcriptional regulation of key cellular processes. Microbiology. 2007, 153 (Pt 11): 3864-3878.PubMedView ArticleGoogle Scholar
- Wiebe M, Rintala E, Tamminen A, Simolin H, Salusjärvi L, Toivari M, Kokkonen J, Kiuru J, Ketola R, Jouhten P, et al: Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions. FEMS Yeast Res. 2008, 8 (1): 140-154. 10.1111/j.1567-1364.2007.00234.x.PubMedView ArticleGoogle Scholar
- Bagnat M, Keränen S, Shevchenko A, Simons K: Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast. Proc Natl Acad Sci USA. 2000, 97 (7): 3254-3259. 10.1073/pnas.060034697.PubMed CentralPubMedView ArticleGoogle Scholar
- Proszynski T, Klemm R, Gravert M, Hsu P, Gloor Y, Wagner J, Kozak K, Grabner H, Walzer K, Bagnat M, et al: A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci USA. 2005, 102 (50): 17981-17986. 10.1073/pnas.0509107102.PubMed CentralPubMedView ArticleGoogle Scholar
- Jin H, McCaffery J, Grote E: Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast. J Cell Biol. 2008, 180 (4): 813-826. 10.1083/jcb.200705076.PubMed CentralPubMedView ArticleGoogle Scholar
- Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact. 2009, 8: 65-10.1186/1475-2859-8-65.PubMed CentralPubMedView ArticleGoogle Scholar
- Shobayashi M, Mitsueda S, Ago M, Fujii T, Iwashita K, Iefuji H: Effects of culture conditions on ergosterol biosynthesis by Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2005, 69 (12): 2381-2388. 10.1271/bbb.69.2381.PubMedView ArticleGoogle Scholar
- Jahnke L, Klein HP: Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae. J Bacteriol. 1983, 155 (2): 488-492.PubMed CentralPubMedGoogle Scholar
- Rosenfeld E, Beauvoit B: Role of the non-respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae. Yeast. 2003, 20 (13): 1115-1144. 10.1002/yea.1026.PubMedView ArticleGoogle Scholar
- Rintala E, Toivari M, Pitkänen J, Wiebe M, Ruohonen L, Penttilä M: Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae. BMC Genomics. 2009, 10: 461-10.1186/1471-2164-10-461.PubMed CentralPubMedView ArticleGoogle Scholar
- Andreasen A, Stier T: Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. J Cell Physiol. 1953, 41 (1): 23-36. 10.1002/jcp.1030410103.PubMedView ArticleGoogle Scholar
- Andreasen A, Stier T: Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. J Cell Physiol. 1954, 43 (3): 271-281. 10.1002/jcp.1030430303.PubMedView ArticleGoogle Scholar
- Bunn HF, Poyton RO: Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev. 1996, 76 (3): 839-885.PubMedGoogle Scholar
- Guan XL, Souza CM, Pichler H, Dewhurst G, Schaad O, Kajiwara K, Wakabayashi H, Ivanova T, Castillon GA, Piccolis M, et al: Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology. Mol Biol Cell. 2009, 20 (7): 2083-2095. 10.1091/mbc.E08-11-1126.PubMed CentralPubMedView ArticleGoogle Scholar
- Eisenkolb M, Zenzmaier C, Leitner E, Schneiter R: A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast. Mol Biol Cell. 2002, 13 (12): 4414-4428. 10.1091/mbc.E02-02-0116.PubMed CentralPubMedView ArticleGoogle Scholar
- Munn AL, Heese-Peck A, Stevenson BJ, Pichler H, Riezman H: Specific sterols required for the internalization step of endocytosis in yeast. Mol Biol Cell. 1999, 10 (11): 3943-3957.PubMed CentralPubMedView ArticleGoogle Scholar
- Bruinenberg P, van Dijken J, Scheffers W: An enzymic analysis of NADPH production and consumption in Candida utilis. J Gen Microbiol. 1983, 129 (4): 965-971.PubMedGoogle Scholar
- González-Siso M, Freire-Picos M, Ramil E, González-Domínguez M, Rodríguez Torres A, Cerdán M: Respirofermentative metabolism in Kluyveromyces lactis: Insights and perspectives. Enzyme Microb Technol. 2000, 26 (9-10): 699-705. 10.1016/S0141-0229(00)00161-7.PubMedView ArticleGoogle Scholar
- Blank L, Sauer U: TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates. Microbiology. 2004, 150 (Pt 4): 1085-1093.PubMedView ArticleGoogle Scholar
- Gancedo C, Serrano R: Energy-yielding metabolism. The Yeasts. Edited by: Harrison JS, Rose AH. 1989, New York: Academic Press, 205-259. 2Google Scholar
- Petrash J, Murthy B, Young M, Morris K, Rikimaru L, Griest T, Harter T: Functional genomic studies of aldo-keto reductases. Chem Biol Interact. 2001, 130-132 (1-3): 673-683. 10.1016/S0009-2797(00)00258-1.PubMedView ArticleGoogle Scholar
- Ingram J, Wood W: Enzymatic basis for D-Arabitol production by Saccharomyces Rouxii. J Bacteriol. 1965, 89: 1186-1194.PubMed CentralPubMedGoogle Scholar
- Marsh L, Rose M: The pathway of cell and nuclear fusion during mating in S. cerevisiae. The Molecular and Cellular Biology of the Yeast Saccharomyces. Edited by: Pringle JR, Broach JR, Jones EW. 1997, Cold Spring Harbor, NY, 827-888.Google Scholar
- Tolstorukov I, Cregg JM: Classical genetics. Methods Mol Biol. 2007, 389: 189-202. 10.1007/978-1-59745-456-8_14.PubMedView ArticleGoogle Scholar
- Epstein C, Waddle J, Hale Wt, Davé V, Thornton J, Macatee T, Garner H, Butow R: Genome-wide responses to mitochondrial dysfunction. Mol Biol Cell. 2001, 12 (2): 297-308.PubMed CentralPubMedView ArticleGoogle Scholar
- Tuttle D, Dunn WJ: Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris. J Cell Sci. 1995, 108 (Pt 1): 25-35.PubMedGoogle Scholar
- Piper M, Daran-Lapujade P, Bro C, Regenberg B, Knudsen S, Nielsen J, Pronk J: Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae. J Biol Chem. 2002, 277 (40): 37001-37008. 10.1074/jbc.M204490200.PubMedView ArticleGoogle Scholar
- Kwast K, Lai L, Menda N, James Dr, Aref S, Burke P: Genomic analyses of anaerobically induced genes in Saccharomyces cerevisiae: functional roles of Rox1 and other factors in mediating the anoxic response. J Bacteriol. 2002, 184 (1): 250-265. 10.1128/JB.184.1.250-265.2002.PubMed CentralPubMedView ArticleGoogle Scholar
- Martíez-Force E, Benítez T: Changes in yeast amino acid pool with respiratory versus fermentative metabolism. Biotechnol Bioeng. 1992, 40 (6): 643-649. 10.1002/bit.260400602.PubMedView ArticleGoogle Scholar
- Krantz M, Nordlander B, Valadi H, Johansson M, Gustafsson L, Hohmann S: Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock. Eukaryot Cell. 2004, 3 (6): 1381-1390. 10.1128/EC.3.6.1381-1390.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- François J, Parrou J: Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev. 2001, 25 (1): 125-145.PubMedView ArticleGoogle Scholar
- Kane S, Roth R: Carbohydrate metabolism during ascospore development in yeast. J Bacteriol. 1974, 118 (1): 8-14.PubMed CentralPubMedGoogle Scholar
- Argüelles J: Physiological roles of trehalose in bacteria and yeasts: a comparative analysis. Arch Microbiol. 2000, 174 (4): 217-224. 10.1007/s002030000192.PubMedView ArticleGoogle Scholar
- Singer M, Lindquist S: Multiple effects of trehalose on protein folding in vitro and in vivo. Mol Cell. 1998, 1 (5): 639-648. 10.1016/S1097-2765(00)80064-7.PubMedView ArticleGoogle Scholar
- Chen Q, Ma E, Behar K, Xu T, Haddad G: Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. J Biol Chem. 2002, 277 (5): 3274-3279. 10.1074/jbc.M109479200.PubMedView ArticleGoogle Scholar
- Cleves A, Cooper D, Barondes S, Kelly R: A new pathway for protein export in Saccharomyces cerevisiae. J Cell Biol. 1996, 133 (5): 1017-1026. 10.1083/jcb.133.5.1017.PubMedView ArticleGoogle Scholar
- Esposito RE, Klapholz S: Meiosis and ascospore development. The Molecular Biology of the Yeast Saccharomyces Life Cycle and Inheritance. Edited by: Strathern JN, Jones EW, Broach JR. 1981, Cold Spring Harbor, NY, 211-287.Google Scholar
- Neiman A: Ascospore formation in the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2005, 69 (4): 565-584. 10.1128/MMBR.69.4.565-584.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Coleman ST, Fang TK, Rovinsky SA, Turano FJ, Moye-Rowley WS: Expression of a glutamate decarboxylase homologue is required for normal oxidative stress tolerance in Saccharomyces cerevisiae. J Biol Chem. 2001, 276 (1): 244-250.PubMedView ArticleGoogle Scholar
- Jang HH, Lee KO, Chi YH, Jung BG, Park SK, Park JH, Lee JR, Lee SS, Moon JC, Yun JW, et al: Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function. Cell. 2004, 117 (5): 625-635. 10.1016/j.cell.2004.05.002.PubMedView ArticleGoogle Scholar
- Trotter EW, Rand JD, Vickerstaff J, Grant CM: The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J. 2008, 412 (1): 73-80. 10.1042/BJ20071634.PubMedView ArticleGoogle Scholar
- Schröder M, Kaufman R: ER stress and the unfolded protein response. Mutat Res. 2005, 569 (1-2): 29-63.PubMedView ArticleGoogle Scholar
- Cox J, Chapman R, Walter P: The unfolded protein response coordinates the production of endoplasmic reticulum protein and endoplasmic reticulum membrane. Mol Biol Cell. 1997, 8 (9): 1805-1814.PubMed CentralPubMedView ArticleGoogle Scholar
- Frand A, Kaiser C: The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum. Mol Cell. 1998, 1 (2): 161-170. 10.1016/S1097-2765(00)80017-9.PubMedView ArticleGoogle Scholar
- Caspi R, Altman T, Dale JM, Dreher K, Fulcher CA, Gilham F, Kaipa P, Karthikeyan AS, Kothari A, Krummenacker M, et al: The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databases. Nucleic Acids Res. 2010, 38 (Database issue): D473-479.PubMed CentralPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.